P2X3 knock-out mice reveal a major sensory role for urothelially released ATP

被引:375
作者
Vlaskovska, M
Kasakov, L
Rong, WF
Bodin, R
Bardini, M
Cockayne, DA
Ford, APDW
Burnstock, G
机构
[1] UCL Royal Free & Univ Coll Med Sch, Auton Neurosci Inst, London NW3 2PF, England
[2] Roche Biosci, Palo Alto, CA 94304 USA
关键词
ATP; P2X(3) receptor; pelvic afferents; immunohistochemistry; capsaicin; knock-out; mouse; urinary bladder;
D O I
10.1523/JNEUROSCI.21-15-05670.2001
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The present study explores the possible involvement of a purinergic mechanism in mechanosensory transduction in the bladder using P2X(3) receptor knock-out (P2X(3)(-/-)) and wildtype control (P2X(3)(+/+)) mice. Immunohistochemistry revealed abundant nerve fibers in a suburothelial plexus in the mouse bladder that are immunoreactive to anti-P2X(3). P2X(3)-positive staining was completely absent in the subepithelial plexus of the P2X(3)(-/-) mice, whereas staining for calcitonin gene-related peptide and vanilloid receptor 1 receptors remained. Using a novel superfused mouse bladder-pelvic nerve preparation, we detected a release of ATP proportional to the extent of bladder distension in both P2X(3)(+/+) and P2X(3)(-/-) mice, although P2X(3)(-/-) bladder had an increased capacity compared with that of the P2X(3)(+/+) bladder. The activity of multifiber pelvic nerve afferents increased progressively during gradual bladder distension (at a rate of 0.1 ml/min). However, the bladder afferents from P2X(3)(-/-) mice showed an attenuated response to bladder distension. Mouse bladder afferents of P2X(3)(+/+), but not P2X(3)(-/-), were rapidly activated by intravesical injections of P2X agonists (ATP or alpha, beta -methylene ATP) and subsequently showed an augmented response to bladder distension. By contrast, P2X antagonists [2', 3'- O-( 2,4,6- trinitrophenyl)-ATP and pyridoxal 5-phosphate 6-azophenyl-2', 4'-disulfonic acid] and capsaicin attenuated distension-induced discharges in bladder afferents. These data strongly suggest a major sensory role for urothelially released ATP acting via P2X(3) receptors on a subpopulation of pelvic afferent fibers.
引用
收藏
页码:5670 / 5677
页数:8
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