Localization of two phylloquinones, QK and QK′, in an improved electron density map of photosystem I at 4-Å resolution

被引:66
作者
Klukas, O
Schubert, WD
Jordan, P
Krauss, N
Fromme, P
Witt, HT
Saenger, W
机构
[1] Free Univ Berlin, Inst Kristallog, D-14195 Berlin, Germany
[2] Tech Univ Berlin, Max Volmer Inst Biophys Chem & Biochem, D-10623 Berlin, Germany
关键词
D O I
10.1074/jbc.274.11.7361
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An improved electron density map of photosystem I from Synechococcus elongatus calculated at 4-Angstrom resolution for the first time reveals a second phylloquinone molecule and thereby completes the set of cofactors constituting the electron transfer system of this iron-sulfur type photosynthetic reaction center: six chlorophyll a, two phylloquinones, and three Fe4S4 clusters. The location of the newly identified phylloquinone pair, the individual plane orientations of these molecules, and the resulting distances to other cofactors of the electron transfer system are discussed and compared with those determined by magnetic resonance techniques.
引用
收藏
页码:7361 / 7367
页数:7
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