3D-reconstruction of blood vessels by ultramicroscopy

被引：73

作者：

Jaehrling, Nina ^{[1
,2
,3
]}

Becker, Klaus ^{[1
,2
]}

Dodt, Hans-Ulrich ^{[1
,2
]}

机构：

[1] Vienna Univ Technol, Inst Solid State Elect, Dept Bioelect, A-1040 Vienna, Austria

[2] Med Univ Vienna, Ctr Brain Res, Vienna, Austria

[3] Carl von Ossietzky Univ Oldenburg, Dept Neurobiol, D-2900 Oldenburg, Germany

来源：

ORGANOGENESIS | 2009年 / 5卷 / 04期

关键词：

3D-reconstruction; blood vessels; cancer; LEA; lectin; microvasculature; morphology; ultramicroscopy; whole mount; 3-DIMENSIONAL VISUALIZATION; BINDING;

D O I：

10.4161/org.5.4.10403

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As recently shown, ultramicroscopy (UM) allows 3D-visualization of even large microscopic structures with mu m resolution. Thus, it can be applied to anatomical studies of numerous biological and medical specimens. We reconstructed the three-dimensional architecture of tomato-lectin (Lycopersicon esculentum) stained vascular networks by UM in whole mouse organs. The topology of filigree branches of the microvasculature was visualized. Since tumors require an extensive growth of blood vessels to survive, this novel approach may open up new vistas in neurobiology and histology, particularly in cancer research.

引用

页码：227 / 230

页数：4

共 13 条

[1]

ahrling N., 2008, MED LASER APPL, V23, P209

[2] Ultramicroscopy: 3D reconstruction of large microscopical specimens [J].