Effects of neocarzinostatin - Chimeric Fab conjugates on the growth of human pancreatic carcinoma xenografts

被引：10

作者：

Otsuji, E

Yamaguchi, T

Tsuruta, H

Yata, Y

Nishi, H

Okamoto, K

Taniguchi, K

Kato, M

Kotani, T

Kitamura, K

Takahashi, T

机构：

[1] First Department of Surgery, Kyoto Pref. University of Medicine, Kamigyo-ku

来源：

BRITISH JOURNAL OF CANCER | 1996年 / 73卷 / 10期

关键词：

pancreatic cancer; neocarzinostatin; monoclonal antibody; chimeric antibody; targeting chemotherapy;

D O I：

10.1038/bjc.1996.227

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Neocarzinostatin (NCS) was bound covalently to human mouse chimeric Fab fragments of MAb A7 (chA7Fab) directed against human pancreatic carcinoma. The anti-tumour effect of chA7Fab-NCS was tested in a nude mouse model on pancreatic carcinoma and compared with A7-NCS or NCS alone. The antitumour effect of chA7Fab-NCS increased in a dose-dependent manner and was significantly greater than either A7-NCS or NCS. Tumour growth was completely suppressed after the administration of chA7Fab-NCS. An enzyme-linked immunosorbent assay with rabbit anti-mouse immunoglobulin was performed to examine the antigenicity of chA7Fab. ChA7Fab had less reactivity with rabbit anti-mouse immunoglobulin than either whole antibody A7 or murine Fab fragments of A7. Thus, chA7Fab-NCS can inhibit human pancreatic cancer growth in an animal and may be useful for targeting chemotherapy to pancreatic cancer in humans.

引用

页码：1178 / 1182

页数：5

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