Urine 8-oxo-7,8-dihydro-2′-deoxyguanosine vs. 5-(hydroxymethyl) uracil as DNA oxidation marker in adriamycin-treated patients

被引：37

作者：

Faure, H

Mousseau, M

Cadet, J

Guimier, C

Tripier, M

Hida, H

Favier, A

机构：

[1] CHU Grenoble, Lab Biochim C, Serv Oncol, F-38043 Grenoble 09, France

[2] Fac Med & Pharm, GREPO, F-38700 La Tronche, France

[3] CEA, SCIB, Dept Rech Fondamentale Mat Condensee, F-38054 Grenoble 9, France

来源：

FREE RADICAL RESEARCH | 1998年 / 28卷 / 04期

关键词：

DNA damage; 5-(hydroxymethyl) uracil; 8-oxo-7,8-dihydro-2 '-deoxyguanosine; adriamycin; human cancer;

D O I：

10.3109/10715769809070806

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We measured the base 5-(hydroxymethyl) uracil (HMUra) and the nucleoside 8-oxo-7,8-dehydro-2'-deoxyguanosine (8-oxo-dGuo) in urine of adriamycin-treated cancer patients. Adriamycin has been shown to generate oxygen free radicals by various mechanisms. HMUra and 8-oxo-dGuo are two known lesions of DNA, produced by oxygen free reaction on thymine and 2'-deoxyguanosine, respectively. HMUra was measured by GC-MS/isotopic dilution and 8-oxo-dGuo by HPLC/EC, both after prepurification by semipreparative HPLC. Here we report the results of a study involving 20 cancer patients treated with flash doses of ADR. We found that urine HMUra is significantly increased (HMUra (nmol/24 h): 80.8 +/- 8.44 vs. 98.7 +/- 6.87; p < 0.01) 24 h after administration of the drug, while 8-oxo-dGuo did not show any significant variation. Urine HMUra seems to be a suitable short-term marker of DNA alterations by oxygen free radicals.

引用

页码：377 / 382

页数：6

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