Shigella protein IpaH9.8 is secreted from bacteria within mammalian cells and transported to the nucleus

被引:61
作者
Toyotome, T
Suzuki, T
Kuwae, A
Nonaka, T
Fukuda, H
Imajoh-Ohmi, S
Toyofuku, T
Hori, M
Sasakawa, C [1 ]
机构
[1] Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol,Div Bacterial Infect, Minato Ku, Tokyo 1088639, Japan
[2] Univ Tokyo, Inst Med Sci, Div Cell Biol & Biochem,Dept Basic Med Sci, Minato Ku, Tokyo 1088639, Japan
[3] Osaka Univ, Grad Sch Med, Dept Internal Med & Therapeut, Suita, Osaka 5650871, Japan
关键词
D O I
10.1074/jbc.M101882200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Various pathogenic bacteria such as Shigella deliver effector proteins into mammalian cells via the type III secretion system. The delivered Shigella effectors have been shown to variously affect host functions required for efficient bacterial internalization into the cells. In the present study, we investigated the IpaH proteins for their ability to be secreted via the type III secretion system and their fate in mammalian cells. Upon incubation in a medium containing Congo red, the bacteria secrete IpaH into the medium, but secretion of IpaH occurs later than that of IpaBCD. Immunofluorescence microscopy indicated that IpaH(9.8) is secreted from intracellular bacteria and transported into the nucleus. On microinjection of the protein, intracellular IpaH(9.8) is accumulated at one place around the nucleus and transported into the nucleus. This movement seems to be dependent on the microtubule network, since nuclear accumulation of IpaH(9.8) is inhibited in cells treated with microtubule-destabilizing agents. In nuclear import assay, IpaH9.8 was efficiently transported into the nucleus, which was completely blocked by treatment with wheat germ agglutinin. The nuclear transport of IpaH(9.8) does not depend on host cytosolic factors but is partially dependent on ATP/GTP, suggesting that, like beta -catenin, IpaH(9.8) secreted from intracellular Shigella can be transported into the nucleus.
引用
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页码:32071 / 32079
页数:9
相关论文
共 53 条
  • [1] ADAM SA, 1992, METHOD ENZYMOL, V219, P97
  • [2] NUCLEAR-PROTEIN IMPORT IN PERMEABILIZED MAMMALIAN-CELLS REQUIRES SOLUBLE CYTOPLASMIC FACTORS
    ADAM, SA
    MARR, RS
    GERACE, L
    [J]. JOURNAL OF CELL BIOLOGY, 1990, 111 (03) : 807 - 816
  • [3] Intracellular traffic of herpes simplex virus glycoprotein gE:: Characterization of the sorting signals required for its trans-Golgi network localization
    Alconada, A
    Bauer, U
    Sodeik, B
    Hoflack, B
    [J]. JOURNAL OF VIROLOGY, 1999, 73 (01) : 377 - 387
  • [4] How to survive in the host:: the Yersinia lesson
    Bleves, S
    Cornelis, GR
    [J]. MICROBES AND INFECTION, 2000, 2 (12) : 1451 - 1460
  • [5] Yop effectors of Yersinia spp. and actin rearrangements
    Bliska, JB
    [J]. TRENDS IN MICROBIOLOGY, 2000, 8 (05) : 205 - 208
  • [6] Binding of the Shigella protein IpaA to vinculin induces F-actin depolymerization
    Bourdet-Sicard, R
    Rüdiger, M
    Jockusch, BM
    Gounon, P
    Sansonetti, PJ
    Van Nhieu, GT
    [J]. EMBO JOURNAL, 1999, 18 (21) : 5853 - 5862
  • [7] Structural and functional diversity in the leucine rich repeat family of proteins
    Buchanan, SGS
    Gay, NJ
    [J]. PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1996, 65 (1-2) : 1 - 44
  • [8] The virulence plasmid pWR100 and the repertoire of proteins secreted by the type III secretion apparatus of Shigella flexneri
    Buchrieser, C
    Glaser, P
    Rusniok, C
    Nedjari, H
    d'Hauteville, H
    Kunst, F
    Sansonetti, P
    Parsot, C
    [J]. MOLECULAR MICROBIOLOGY, 2000, 38 (04) : 760 - 771
  • [9] ANALYSIS OF GENE-CONTROL SIGNALS BY DNA-FUSION AND CLONING IN ESCHERICHIA-COLI
    CASADABAN, MJ
    COHEN, SN
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1980, 138 (02) : 179 - 207
  • [10] Enteropathogenic Escherichia coli (EPEC) attachment to epithelial cells:: exploiting the host cell cytoskeleton from the outside
    Celli, J
    Deng, WY
    Finlay, BB
    [J]. CELLULAR MICROBIOLOGY, 2000, 2 (01) : 1 - 9