Detection of Nickel in Fish Organs with a Two-Photon Fluorescent Probe

被引：25

作者：

Kang, Min Young ^{[3
]}

Lim, Chang Su ^{[3
]}

Kim, Hyun Soo ^{[3
]}

Seo, Eun Won ^{[1
]}

Kim, Hwan Myung ^{[1
]}

Kwon, Ohyun ^{[2
]}

Cho, Bong Rae ^{[3
]}

机构：

[1] Ajou Univ, Div Energy Syst Res, Suwon 443749, South Korea

[2] Samsung Adv Inst Technol, Suwon, South Korea

[3] Korea Univ, Dept Chem, Seoul 136713, South Korea

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2012年 / 18卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

fish; fluorescent probes; live tissue; nickel; two-photon microscopy; MICROSCOPY; SENSORS; COMPLEX; IONS;

D O I：

10.1002/chem.201103191

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Molecular imaging by two-photon microscopy (TPM) has become indispensable to the study of biology/medicine owing to its capability of imaging deep inside intact tissues. To make TPM a more-versatile tool, a large variety of two-photon probes are needed. Herein, we report a new two-photon fluorescent probe (ANi2) that can be excited by 750 nm femtosecond pulses and detect Ni2+ ions in fresh fish organs at 90-175 mu m depth without interference from the pH value or from other biologically relevant species through the use of TPM. TPM images of fish organs labeled with ANi2 revealed that Ni2+ ions accumulate in fish organs in the order: kidney > heart > gill >= liver. Moreover, a linear relationship was found between the two-photon-excited fluorescence (TPEF) and the inductively coupled plasma mass spectrometry intensities (ICP-MS), thereby allowing the quantitative measurement of Ni2+ ions in live tissue.

引用

页码：1953 / 1960

页数：8

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