A Turn-On Fluorescent Sensor for Detecting Nickel in Living Cells

被引：209

作者：

Dodani, Sheel C.

He, Qiwen

Chang, Christopher J. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 50期

关键词：

STRUCTURE/FUNCTION RELATIONSHIPS; TRANSITION-METALS; CRYSTAL-STRUCTURE; ESCHERICHIA-COLI; AQUEOUS-SOLUTION; BINDING-PROTEIN; NI2+ ION; NIKR; CHEMOSENSORS; CHEMISTRY;

D O I：

10.1021/ja906500m

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present the synthesis and properties of Nickelsensor-1 (NS1), a new water-soluble, turn-on fluorescent sensor that is capable of selectively responding to Ni2+ in aqueous solution and in living cells. NS1 combines a BODIPY chromophore and a mixed N/O/S receptor to provide good selectivity for Ni2+ over a range of biologically abundant metal ions in aqueous solution. In addition to these characteristics, confocal microscopy experiments further show that NS1 can be delivered into living cells and report changes in intracellular Ni2+ levels in a respiratory cell model.

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页码：18020 / +

页数：3

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