Modern Pathology Methods for Neural Investigations

被引：8

作者：

Hale, Sarah L. ^{[2
]}

Andrews-Jones, Lydia ^{[1
]}

Jordan, William H. ^{[3
]}

Jortner, Bernard S. ^{[4
]}

Boyce, Rogely W. ^{[5
]}

Boyce, John T. ^{[5
]}

Switzer, Robert C., III ^{[6
]}

Butt, Mark T. ^{[7
]}

Garman, Robert H. ^{[8
]}

Jensen, Karl ^{[9
]}

Krinke, Georg ^{[2
]}

Little, Peter B. ^{[10
]}

机构：

[1] Allergan, Drug Safety Evaluat, Irvine, CA 92612 USA

[2] Covance Labs Inc, Vienna, VA USA

[3] Vet Path Serv Inc, Mason, OH USA

[4] Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Blacksburg, VA 24061 USA

[5] WIL Res Labs, Ashland, OH USA

[6] NeuroScience Associates, Knoxville, TN USA

[7] Tox Path Specialists LLC, Walkersville, MD USA

[8] Consultants Vet Pathol Inc, Murrysville, PA USA

[9] US EPA, Res Triangle Pk, NC 27711 USA

[10] Charles River Labs PAI, Durham, NC USA

来源：

TOXICOLOGIC PATHOLOGY | 2011年 / 39卷 / 01期

关键词：

nervous system sampling; neurohistology; stereology; RAPID METHOD; NEUROTOXICITY; INHALATION; RATS;

D O I：

10.1177/0192623310394213

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

This session at the 2010 joint symposium of the Society of Toxicologic Pathology (STP) and the International Federation of Societies of Toxicologic Pathologists (IFSTP) explored modern neuropathology methods for assessing the neurotoxicologic potential of xenobiotics. Conventional techniques to optimally prepare and evaluate the central and peripheral neural tissues while minimizing artifact were reviewed, and optimal schemes were set forth for evaluation of the nervous system during both routine (i.e., general toxicity) studies and enhanced (i.e., specialized neurotoxicity) studies. Stereology was introduced as the most appropriate means of examining the possible impact of toxicants on neural cell numbers. A focused discussion on brain sampling took place among a panel of expert neuroscientists (anatomists and pathologists) and the audience regarding the proper balance between sufficient sampling and cost-and time-effectiveness of the analysis. No consensus was reached on section orientation (coronal sections of both sides vs. a parasagittal longitudinal section with several unilateral hemisections from the contralateral side), but most panelists favored sampling at least 8 sections (or approximately double to triple the current complement) in routine toxicity studies.

引用

页码：52 / 57

页数：6

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