The putative protein 6 of the severe acute respiratory syndrome-aassociated coronavirus: Expression and functional characterization

被引：28

作者：

Geng, H

Liu, YM

Chan, WS

Lo, AWI

Au, DMY

Waye, MMY

Ho, YY

机构：

[1] Chinese Univ Hong Kong, Dept Biochem, Shatin, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Anat & Cellular Pathol, Shatin, Hong Kong, Peoples R China

[3] Chinese Univ Hong Kong, Croucher Lab Human Genom, Shatin, Hong Kong, Peoples R China

[4] Century Biotech Ltd, Hong Kong, Hong Kong, Peoples R China

来源：

FEBS LETTERS | 2005年 / 579卷 / 30期

关键词：

severe acute respiratory syndrome; coronavirus; SARS-CoV; ORF6; SARS; 6; protein;

D O I：

10.1016/j.febslet.2005.11.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：6763 / 6768

页数：6

相关论文

共 22 条

[1] Persistent infection of SARS coronavirus in colonic cells in vitro [J].

Chan, PKS ;

To, KF ;

Lo, AWI ;

Cheung, JLK ;

Chu, I ;

Au, FWL ;

Tong, JHM ;

Tam, JS ;

Sung, JJY ;

Ng, HK .

JOURNAL OF MEDICAL VIROLOGY, 2004, 74 (01) :1-7

[2]

CHAN WS, 2005, MOD PATHOL

[3] Genomic characterisation of the severe acute respiratory syndrome coronavirus of Amoy Gardens outbreak in Hong Kong [J].

Chim, SSC ;

Tsui, SKW ;

Chan, KCA ;

Au, TCC ;

Hung, ECW ;

Tong, YK ;

Chiu, RWK ;

Ng, EKO ;

Chan, PKS ;

Chu, CM ;

Sung, JJY ;

Tam, JS ;

Fung, KP ;

Waye, MMY ;

Lee, CY ;

Yuen, KY ;

Lo, YMD .

LANCET, 2003, 362 (9398) :1807-1808

[4] Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirus [J].

Fielding, BC ;

Tan, YJ ;

Shuo, S ;

Tan, THP ;

Ooi, EE ;

Lim, SG ;

Hong, WJ ;

Goh, PY .

JOURNAL OF VIROLOGY, 2004, 78 (14) :7311-7318

[5] A YEAST RNA-BINDING PROTEIN SHUTTLES BETWEEN THE NUCLEUS AND THE CYTOPLASM [J].

FLACH, J ;

BOSSIE, M ;

VOGEL, J ;

CORBETT, A ;

JINKS, T ;

WILLINS, DA ;

SILVER, PA .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (12) :8399-8407

[6] The 3a protein of severe acute respiratory syndrome-associated coronavirus induces apoptosis in Vero E6 cells [J].

Law, PTW ;

Wong, CH ;

Au, TCC ;

Chuck, CP ;

Kong, SK ;

Chan, PKS ;

To, KF ;

Lo, AWI ;

Chan, JYW ;

Suen, YK ;

Chan, HYE ;

Fung, KP ;

Waye, MMY ;

Sung, JJY ;

Lo, YMD ;

Tsui, SKW .

JOURNAL OF GENERAL VIROLOGY, 2005, 86 :1921-1930

[7] A major outbreak of severe acute respiratory syndrome in Hong Kong [J].

Lee, N ;

Hui, D ;

Wu, A ;

Chan, P ;

Cameron, P ;

Joynt, GM ;

Ahuja, A ;

Yung, MY ;

Leung, CB ;

To, KF ;

Lui, SF ;

Szeto, CC ;

Chung, S ;

Sung, JJY .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1986-1994

[8] Ubiquitin-dependent proteolysis of cyclin D1 is associated with coxsackievirus-induced cell growth arrest [J].

Luo, HL ;

Zhang, JC ;

Dastvan, F ;

Yanagawa, B ;

Reidy, MA ;

Zhang, HFM ;

Yang, DC ;

Wilson, JE ;

McManus, BM .

JOURNAL OF VIROLOGY, 2003, 77 (01) :1-9

[9] THE JELLYFISH GREEN FLUORESCENT PROTEIN - A NEW TOOL FOR STUDYING ION-CHANNEL EXPRESSION AND FUNCTION [J].

MARSHALL, J ;

MOLLOY, R ;

MOSS, GWJ ;

HOWE, JR ;

HUGHES, TE .

NEURON, 1995, 14 (02) :211-215

[10] HTLV-1 p12I protein enhances STAT5 activation and decreases the interleukin-2 requirement for proliferation of primary human peripheral blood mononuclear cells [J].

Nicot, C ;

Mulloy, JC ;

Ferrari, MG ;

Johnson, JM ;

Fu, KS ;

Fukumoto, R ;

Trovato, R ;

Fullen, J ;

Leonard, WJ ;

Franchini, G .

BLOOD, 2001, 98 (03) :823-829