Fast Resonance Assignment and Fold Determination of Human Superoxide Dismutase by High-Resolution Proton-Detected Solid-State MAS NMR Spectroscopy

被引：137

作者：

Knight, Michael J. ^{[1
]}

Webber, Amy L. ^{[1
]}

Pell, Andrew J. ^{[1
]}

Guerry, Paul ^{[1
]}

Barbet-Massin, Emeline ^{[1
]}

Bertini, Ivano ^{[2
,3
]}

Felli, Isabella C. ^{[2
,3
]}

Gonnelli, Leonardo ^{[2
,3
]}

Pierattelli, Roberta ^{[2
,3
]}

Emsley, Lyndon ^{[1
]}

Lesage, Anne ^{[1
]}

Herrmann, Torsten ^{[1
]}

Pintacuda, Guido ^{[1
]}

机构：

[1] Univ Lyon, Ecole Normale Super Lyon, Ctr RMN Tres Hauts Champs, CNRS,UMR 5280, F-69100 Villeurbanne, France

[2] Univ Florence, Dept Chem Ugo Schiff, I-50019 Sesto Fiorentino Fi, Italy

[3] Univ Florence, Magnet Resonance Ctr CERM, I-50019 Sesto Fiorentino Fi, Italy

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 49期

关键词：

1H detection; NMR spectroscopy; protein structure; structural constraints; PROTEIN-STRUCTURE DETERMINATION; 3D STRUCTURE DETERMINATION; PERDEUTERATED PROTEINS; MEMBRANE-PROTEINS; LARGER PROTEINS; WILD-TYPE; SPECTRA; C-13; RESTRAINTS; COHERENCE;

D O I：

10.1002/anie.201106340

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Re-protonation is key: A combination of a high magnetic field (1 GHz) and ultra-fast magic-angle spinning (60 kHz) allows easy detection of NMR spectra revealing details of secondary and tertiary structures of medium-sized proteins. The technique was applied to the 153-residue microcrystalline Zn II-loaded superoxide dismutase (ZnII-SOD) fully [ 2H,13C,15N]-labeled and 100% re-protonated at the exchangeable sites. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：11697 / 11701

页数：5

共 41 条

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Optimum levels of exchangeable protons in perdeuterated proteins for proton detection in MAS solid-state NMR spectroscopy [J].