Characterization of a naturally occurring class D β-lactamase from Achromobacter xylosoxidans

被引：51

作者：

Doi, Yohei ^{[1
,2
]}

Poirel, Laurent ^{[1
]}

Paterson, David L. ^{[2
,3
]}

Nordmann, Patrice ^{[1
]}

机构：

[1] Univ Paris 11, Serv Bacteriol Virol, Hop Bicetre,U Emerging Resistance Antibiot 914, INSERM,AP HP,Fac Med Paris Sud, F-94275 K Bicetre, France

[2] Univ Pittsburgh, Med Ctr, Div Infect Dis, Pittsburgh, PA USA

[3] Univ Queensland, Royal Brisbane & Womens Hosp, Brisbane, Qld, Australia

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2008年 / 52卷 / 06期

关键词：

D O I：

10.1128/AAC.01463-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A chromosomally encoded class D beta-lactamase, OXA-114, was characterized from Achromobacter xylosoxidans strain CIP69598. beta-Lactamase OXA-114 shared 56% amino acid identity with the naturally occurring class D beta-lactamase of Burkholderia cenocepacia and 42% identity with the acquired oxacillinases OXA-9 and OXA-18. OXA-114 has a narrow-spectrum hydrolysis profile, although it includes imipenem, at a very low level. PCR and sequencing revealed that bla(OXA114)-like genes were identified in all A. xylosoxidans strains tested (n = 5), indicating that this beta-lactamase is naturally occurring in that species. Induction experiments with imipenem and cefoxitin did not show inducibility of bla(OXA)-114 expression.

引用

页码：1952 / 1956

页数：5

共 22 条

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