Antimicrobial activity of human beta-defensins and induction by Francisella

被引：39

作者：

Han, SuHua ^{[1
]}

Bishop, Barney M. ^{[2
]}

van Hoek, Monique L. ^{[1
]}

机构：

[1] George Mason Univ, Natl Ctr Biodef & Infect Dis, Dept Mol & Microbiol, Manassas, VA 20110 USA

[2] George Mason Univ, Dept Chem & Biochem, Manassas, VA 20110 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 371卷 / 04期

关键词：

Francisella tularensis; live vaccine strain; Francisella novicida; beta-defensin; antimicrobial peptide; A549; lung epithelial cell;

D O I：

10.1016/j.bbrc.2008.04.092

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of human beta-defensins hBD-1, hBD-2, and hBD-3 to exert direct in vitro antimicrobial effects was evaluated using Francisella tularensis Live Vaccine Strain (LVS) and Francisella novicida. While hBD-2 showed some antimicrobial activity in these assays, only hBD-3 demonstrated significant potency against Francisella. Francisella tularensis LVS infection induced elevated levels of hBD-2 mRNA in human airway epithelial (A549) cells, while having no significant impact on the levels of hBD-3 and only a moderate effect on the level of hBD-1 mRNA. Francisella infection avoided stimulating the production of the most potent anti-Francisella host peptide, hBD-3, in A549 cells, although hBD-3 is stimulated by other treatments. The differential induction of beta-defensins in Francisella infected lung epithelial cells suggests a complex dynamic in the expression of antimicrobial peptides and the innate immune response. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：670 / 674

页数：5

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