Human and rat dipeptidyl peptidase III:: Biochemical and mass spectrometric arguments for similarities and differences

Dipeptidyl peptidase (DPP tit) was purified from rat and human erythrocytes using an identical procedure. Electrophoretic analyses revealed the same molecular size and pi for both enzymes. The molecular mass of the human enzyme, measured by matrix-assisted laser desorption/ionization MS, was 82500 +/- 60 Da, Its tryptic peptide mass profile was determined using the same technique, and the amino acid sequence of two internal peptides was obtained by tandem MS and Edman degradation. A search of databases revealed a high similarity between the human eryrthrocyte and rat liver DPP III: 21 matches out of 34 detected peptides were found, covering 40% of the total sequence. Matched peptides included the peptide harboring the characteristic HELLGH sequence motif, and a stretch of 19 identical amino acids, containing Glu, a putative ligand of active site zinc. Both enzymes preferred Arg-Arg-2-naphthylamide, and were activated by micromolar Co2+, differing in their pH optima and k(cat)/K-m, Zn2+ ions, sulfhydryl reagents, andaminopeptidase inhibitors, especially probestin, inhibited the rat DPP III more potently, The two enzymes showed the highest affinity for angiotensin III (K-i < 1 <mu>M) and a preference for a hydrophobic residue at the P-1' site, However, significant differences in the binding constants for several peptides indicated non-identity in the active site topography of human and rat erythrocyte DPP III.