Rapid identification and differentiation of pathogenic clostridia in gas gangrene by polymerase chain reaction based on the 16S-23S rDNA spacer region

被引:31
作者
Sasaki, Y
Yamamoto, K
Kojima, A
Norimatsu, M
Tamura, Y
机构
[1] Natl Vet Assay Lab, Kokubunji, Tokyo 1858511, Japan
[2] Inst Anim Hlth, Newbury RG20 7NN, Berks, England
关键词
D O I
10.1053/rvsc.2000.0431
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In cattle, sheep, and other ruminants, clostridial myonecrosis (gas gangrene) is mostly caused by Clostridium chauvoei, C septicum, C novyi and C sordellii. A polymerase chain reaction (PCR) system using common primers designed from multiple alignment of the 16S rRNA and 23S rRNA genes of Clostridium species was developed to identify pathogenic clostridia. The PCR was performed with total DNA from 26 strains which included seven different Clostridia species. These bacteria were differentiated at species level by the different pen product patterns. To characterise the 16S-23S rDNA spacer regions of these clostridia further, most PCR products of these bacteria were sequenced. The smallest PCR products of each bacterium represented the fundamental 16S-23S rDNA spacer region; larger PCR products of each bacterium were caused by insertion sequences, i.e. tRNA gene sequences. The authors' observations indicate that the PCR patterns of the 16S-23S rDNA spacer regions have the potential to be used as an identification marker of pathogenic clostridia in gas gangrene. (C) 2000 Harcourt Publishers Ltd.
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收藏
页码:289 / 294
页数:6
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