Virus detection protocols for West Nile virus in vertebrate and mosquito specimens

被引：63

作者：

Kauffman, EB ^{[1
]}

Jones, SA ^{[1
]}

Dupuis, AP ^{[1
]}

Ngo, KA ^{[1
]}

Bernard, KA ^{[1
]}

Kramer, LD ^{[1
]}

机构：

[1] New York State Dept Hlth, Arbovirus Labs, Wadsworth Ctr, Slingerlands, NY 12159 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 08期

关键词：

D O I：

10.1128/JCM.41.8.3661-3667.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The recent outbreaks of West Nile virus (WNV) infection in the northeastern United States and other regions of the world have made it essential to develop efficient, sensitive, and rapid protocols for virus surveillance. Laboratory testing is the backbone of any surveillance program. Protocols to detect the presence of WNV have been refined since 1999 for sensitivity, speed, efficiency, and specificity. This paper presents the protocols currently used by the New York State Department of Health to handle vertebrate and mosquito specimens that have been submitted for WNV testing to the Arbovirus Laboratories of the Wadsworth Center.

引用

页码：3661 / 3667

页数：7

共 15 条

[1] Isolation of West Nile virus from mosquitoes, crows, and a Cooper's hawk in Connecticut [J].

Anderson, JF ;

Andreadis, TG ;

Vossbrinck, CR ;

Tirrell, S ;

Wakem, EM ;

French, RA ;

Garmendia, AE ;

Van Kruiningen, HJ .

SCIENCE, 1999, 286 (5448) :2331-2333

[2]

Bernard KA, 2001, EMERG INFECT DIS, V7, P679

[3]

*CDCP, 2000, MMWR-MORBID MORTAL W, V49, P714

[4]

*CDCP, 2002, MMWR-MORBID MORTAL W, V51, P1026

[5] Detection by enzyme-linked immunosorbent assay of antibodies to West Nile virus in birds [J].

Ebel, GD ;

Dupuis, AP ;

Nicholas, D ;

Young, D ;

Maffei, J ;

Kramer, LD .

EMERGING INFECTIOUS DISEASES, 2002, 8 (09) :979-982

[6] Detection of arboviral RNA directly from mosquito homogenates by reverse-transcription-polymerase chain reaction [J].

Huang, C ;

Slater, B ;

Campbell, W ;

Howard, J ;

White, D .

JOURNAL OF VIROLOGICAL METHODS, 2001, 94 (1-2) :121-128

[7] Rapid detection of West Nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay [J].

Lanciotti, RS ;

Kerst, AJ ;

Nasci, RS ;

Godsey, MS ;

Mitchell, CJ ;

Savage, HM ;

Komar, N ;

Panella, NA ;

Allen, BC ;

Volpe, KE ;

Davis, BS ;

Roehrig, JT .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (11) :4066-4071

[8] Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States [J].

Lanciotti, RS ;

Roehrig, JT ;

Deubel, V ;

Smith, J ;

Parker, M ;

Steele, K ;

Crise, B ;

Volpe, KE ;

Crabtree, MB ;

Scherret, JH ;

Hall, RA ;

MacKenzie, JS ;

Cropp, CB ;

Panigrahy, B ;

Ostlund, E ;

Schmitt, B ;

Malkinson, M ;

Banet, C ;

Weissman, J ;

Komar, N ;

Savage, HM ;

Stone, W ;

McNamara, T ;

Gubler, DJ .

SCIENCE, 1999, 286 (5448) :2333-2337

[9] Real-time PCR in virology [J].

Mackay, IM ;

Arden, KE ;

Nitsche, A .

NUCLEIC ACIDS RESEARCH, 2002, 30 (06) :1292-1305

[10]

Melnick J L, 1979, Prog Med Virol, V25, P160

← 1 2 →