Development, Characterization, and Application of Monoclonal Antibodies against Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein

被引：13

作者：

Das, Dipankar ^{[1
]}

Kammila, Sriram ^{[1
]}

Suresh, Mavanur R. ^{[1
]}

机构：

[1] Univ Alberta, Fac Pharm & Pharmaceut Sci, Edmonton, AB T6G 2N8, Canada

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2010年 / 17卷 / 12期

关键词：

LINKED-IMMUNOSORBENT-ASSAY; SARS-COV; ANTIGEN; SERA;

D O I：

10.1128/CVI.00293-10

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Five monoclonal antibodies (MAbs) against recombinant nucleocapsid protein (NP) of severe acute respiratory syndrome (SARS)-causing coronavirus (CoV) were developed by hybridoma technology. Epitope mapping by Western blotting showed that these anti-SARS-CoV NP MAbs bind to distinct domains of NP. These anti-SARS-CoV NP MAbs, with their high specificity, are potentially ideal candidates for developing early and sensitive diagnostic assays for SARS-CoV.

引用

页码：2033 / 2036

页数：4

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