Development of an up-converting phosphor technology-based 10-channel lateral flow assay for profiling antibodies against Yersinia pestis

被引：73

作者：

Hong, Wenyan ^{[1
]}

Huang, Lihua ^{[2
]}

Wang, Haoran ^{[1
]}

Qu, Jianfeng ^{[2
]}

Guo, Zhaobiao ^{[1
]}

Xie, Chengke ^{[2
]}

Zhu, Ziwen ^{[1
]}

Zhang, Youbao ^{[2
]}

Du, Zongmin ^{[1
]}

Yan, Yanfeng ^{[1
]}

Zheng, Yan ^{[3
]}

Huang, Huijie ^{[2
]}

Yang, Ruifu ^{[1
]}

Zhou, Lei ^{[1
]}

机构：

[1] Beijing Inst Microbiol & Epidemiol, Lab Analyt Microbiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, Shanghai 201800, Peoples R China

[3] Shanghai Kerune Phosphor Technol Co Ltd, Shanghai 201108, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2010年 / 83卷 / 02期

基金：

北京市自然科学基金;

关键词：

Up-converting phosphor; Lateral flow assay; Multi-detection; Antibodies profiling; Yersinia pestis; QUANTITATIVE DETECTION; RAPID-DETECTION; IMMUNOASSAY; ANTIGEN; IMMUNOSENSORS; MICROARRAYS; RESPONSES; VACCINE; SERUM;

D O I：

10.1016/j.mimet.2010.08.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, a 10-channel up-converting phosphor technology-based lateral flow (TC-UPT-LF) assay was developed to profile antibodies against Yersinia pestis. Ten expressed Y. pestis proteins were covalently conjugated with an up-converting phosphor particle to develop double-antigen sandwich immunochromatographic strips to detect corresponding antibodies. After optimization one by one, each strip was integrated into a TC-UPT-LF disc for simultaneously detection of different antibodies. A scanning biosensor was also developed to acquire the results. The performance of the TC-UPT-LF assay was evaluated by using standard samples and plague monkey serum samples. Fifty-one patient serum samples were detected by the TC-UPT-LF assay. The TC-UPT-LF disc could be stable for 10 days at 37 degrees C with an average CV of 10.3%. Its sensitivity and qualitative results are comparable to those of ELISA. Its linearity fitting coefficient of determination (R-2) for different antibody detection is between 0.93 and 0.99. Besides F1 antibody, the LcrV and YopD antibodies also showed higher positive ratio than the other seven antibodies, as 100% (13/13) and 92% (12/13) in monkey sera and 86.3% (44/51) and 66.7% (34/51) in patient sera, respectively. It is suggested that the TC-UPT-LF assay has been successfully developed for multi-detection and LcrV and YopD can be the potential diagnostic markers of the plague. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：133 / 140

页数：8

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