Fast DNA-based identification of the black truffle Tuber melanosporum with direct PCR and species-specific primers

被引：31

作者：

Bonito, Gregory ^{[1
]}

机构：

[1] Duke Univ, Dept Biol, Durham, NC 27708 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2009年 / 301卷 / 02期

关键词：

Tuber melanosporum; truffles; DNA barcoding; direct PCR; species-specific primers;

D O I：

10.1111/j.1574-6968.2009.01812.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A quick and sensitive DNA-based assay for identifying the black truffle species Tuber melanosporum is presented. Both direct PCR and species-specific primers are used to amplify a phylogenetically informative region of T. melanosporum rDNA. This method was successfully used to screen fresh and frozen T. melanosporum fruitbodies, and could be modified for the molecular detection of other truffle and mushroom species.

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收藏

页码：171 / 175

页数：5

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