Molecular markers for the identification of the ectomycorrhizal fungus Tuber borchii

被引：30

作者：

Bertini, L

Agostini, D

Potenza, L

Rossi, I

Zeppa, S

Zambonelli, A

Stocchi, V

机构：

[1] Univ Urbino, Ist Chim Biol Giorgio Fornaini, I-61029 Urbino, PS, Italy

[2] Univ Bologna, Dipartimento Protez & Valorizzaz Agroalimentare, I-40126 Bologna, Italy

来源：

NEW PHYTOLOGIST | 1998年 / 139卷 / 03期

关键词：

RAPD/PCR fingerprint; SCAR marker; Tuber borchii Vittad; ectomycorrhiza;

D O I：

10.1046/j.1469-8137.1998.00203.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Five species of white truffle were classified using PCR-based techniques. RAPD (random amplified polymorphic DNA) fingerprints and specific pairs of primers were used. A RAPD fragment was constant in Tuber borchii Vittad. isolates and polymorphic among the other species. Two molecular markers specific for T. borchii were developed from the sequence of the non-polymorphic RAPD fragment and from regions flanking the 5'-3' ends of a truffle gene. These markers were applied in the identification of T. borchii fruit bodies, mycelia and mycorrhizas, allowing us to monitor the development of this fungus during its entire life cycle.

引用

页码：565 / 570

页数：6

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