Gait Analysis in Normal and Spinal Contused Mice Using the TreadScan System

被引:75
作者
Beare, Jason E. [1 ,2 ]
Morehouse, Johnny R. [1 ,3 ]
DeVries, William H. [1 ,3 ]
Enzmann, Gaby U. [1 ,3 ]
Burke, Darlene A. [1 ,3 ]
Magnuson, David S. K. [1 ,2 ,3 ]
Whittemore, Scott R. [1 ,2 ,3 ]
机构
[1] Univ Louisville, Kentucky Spinal Cord Injury Res Ctr, Louisville, KY 40292 USA
[2] Univ Louisville, Dept Anat Sci & Neurobiol, Louisville, KY 40292 USA
[3] Univ Louisville, Dept Neurol Surg, Louisville, KY 40292 USA
关键词
behavioral assessments; locomotor function; spinal cord injury (SCI); CORD-INJURY; FUNCTIONAL RECOVERY; OVERGROUND LOCOMOTION; TREADMILL LOCOMOTION; PARAPLEGIC MICE; GRADED MODEL; MOUSE; OUTCOMES; RAT; STRIDE;
D O I
10.1089/neu.2009.0914
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Advances in spinal cord injury (SCI) research are dependent on quality animal models, which in turn rely on sensitive outcome measures able to detect functional differences in animals following injury. To date, most measurements of dysfunction following SCI rely either on the subjective rating of observers or the slow throughput of manual gait assessment. The present study compares the gait of normal and contusion-injured mice using the TreadScan (R) system. TreadScan utilizes a transparent treadmill belt and a high-speed camera to capture the footprints of animals and automatically analyze gait characteristics. Adult female C57B1/6 mice were introduced to the treadmill prior to receiving either a standardized mild, moderate, or sham contusion spinal cord injury. TreadScan gait analyses were performed weekly for 10 weeks and compared with scores on the Basso Mouse Scale (BMS). Results indicate that this software successfully differentiates sham animals from injured animals on a number of gait characteristics, including hindlimb swing time, stride length, toe spread, and track width. Differences were found between mild and moderate contusion injuries, indicating a high degree of sensitivity within the system. Rear track width, a measure of the animal's hindlimb base of support, correlated strongly both with spared white matter percentage and with terminal BMS. TreadScan allows for an objective and rapid behavioral assessment of locomotor function following mild-moderate contusive SCI, where the majority of mice still exhibit hindlimb weight support and plantar paw placement during stepping.
引用
收藏
页码:2045 / 2056
页数:12
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