C. Diff Quik Chek Complete Enzyme Immunoassay Provides a Reliable First-Line Method for Detection of Clostridium difficile in Stool Specimens

被引：88

作者：

Quinn, Criziel D. ^{[1
]}

Sefers, Susan E. ^{[1
]}

Babiker, Wisal ^{[3
]}

He, Ying ^{[1
]}

Alcabasa, Romina ^{[3
]}

Stratton, Charles W. ^{[1
,2
]}

Carroll, Karen C. ^{[3
]}

Tang, Yi-Wei ^{[1
,2
]}

机构：

[1] Vanderbilt Univ, Dept Pathol, Sch Med, Nashville, TN 37232 USA

[2] Vanderbilt Univ, Dept Med, Sch Med, Nashville, TN 37232 USA

[3] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21287 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 02期

关键词：

REAL-TIME PCR; PREMIER TOXIN-A; MULTICENTER EVALUATION; CYTOTOXICITY ASSAY; DIAGNOSIS; CULTURE; PERFORMANCE; INFECTION; DIARRHEA; ANTIGEN;

D O I：

10.1128/JCM.01614-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated a single membrane device assay for simultaneously detecting both Clostridium difficile glutamate dehydrogenase (GDH) and toxin A/B antigens against a standard that combines two PCR assays and cytotoxigenic culture. Results showing dual GDH and toxin A/B antigen positives and negatives can be reported immediately as true positives and negatives, respectively. Specimens with discrepant results for GDH and toxins A/B, which comprised 13.2% of the specimens, need to be retested.

引用

页码：603 / 605

页数：3

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