Expression of NAD+-dependent formate dehydrogenase in Enterobacter aerogenes and its involvement in anaerobic metabolism and H2 production

被引：20

作者：

Lu, Yuan ^{[1
]}

Zhao, Hongxin ^{[1
]}

Zhang, Chong ^{[1
]}

Lai, Qiheng ^{[1
]}

Wu, Xi ^{[1
]}

Xing, Xin-Hui ^{[1
]}

机构：

[1] Tsinghua Univ, Inst Biochem Engn, Dept Chem Engn, Beijing 100084, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Anaerobic fermentation; Enterobacter aerogens; Formate dehydrogenase; Hydrogen production; Metabolic analysis; NADH regeneration; AEROBIC FLUORESCENCE RECOVERY; HYDROGEN-PRODUCTION; ESCHERICHIA-COLI; BIOHYDROGEN PRODUCTION; CARBON; NADH;

D O I：

10.1007/s10529-009-0036-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An expression system for NAD(+)-dependent formate dehydrogenase gene (fdh1), from Candida boidinii, was constructed and cloned into Enterobacter aerogenes IAM1183. With the fdh1 expression, the total H-2 yield was attributed to a decrease in activity of the lactate pathway and an increase of the formate pathway flux due to the NADH regeneration. Analysis of the redox state balance and ethanol-to-acetate ratio in the fdhl-expressed strain showed that increased reducing power arose from the reconstruction of NADH regeneration pathway from formate thereby contributing to the improved H-2 production.

引用

页码：1525 / 1530

页数：6

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