Analysis of LMW RNA profiles of Frankia strains by staircase electrophoresis

被引：18

作者：

Velázquez, E

Cervantes, E

Igual, JM

Peix, A

Mateos, PF

Benamar, S

Moiroud, A

Wheeler, CT

Dawson, J

Labeda, D

Rodríguez-Barrueco, C

Martínez-Molina, E

机构：

[1] CSIC, IRNA, Salamanca 37080, Spain

[2] Dept Microbiol & Genet, Edificio Departamental, Salamanca, Spain

[3] Ecole Normale Super, Lab Biol & Physiol Vegetales & Forestieres, Bensouda, Morocco

[4] Univ Lyon 1, F-69622 Villeurbanne, France

[5] Univ Glasgow, Inst Biomed & Life Sci, Div Biochem & Mol Biol, Glasgow, Lanark, Scotland

[6] Univ Illinois, Dept Nat Ressources & Environm Sci, Plant Sci Lab 1005A, Urbana, IL 61801 USA

[7] Natl Ctr Agr Utilizat Res, Peoria, IL USA

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 1998年 / 21卷 / 04期

关键词：

Frankia; LMW RNA; nitrogen fixation; bacterial taxonomy;

D O I：

10.1016/S0723-2020(98)80066-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An optimized technique of polyacrylamide gel electrophoresis, Staircase Electrophoresis (SCE), was applied to determine the stable Low Molecular Weight RNA (LMW RNA) profiles of 25 Frankia strains from diverse geographic origins and host specificity groups as well as species from other actinomycete genera. Application of the technique permits the rapid identification of Frankia strains and their differentiation from other actinomycetes. The isolates used in this study were grouped in eight clusters, each comprising strains with identical LMW RNA profiles. Comparison of these results with others obtained from DNA sequences or DNA hybridization methods suggest a high degree of complexity in the genus Frankia. Application of SCE to profile LMW RNA should in the future facilitate biodiversity studies of Frankia and discrimination of new species.

引用

页码：539 / 545

页数：7

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