Universal hybridization using LNA (locked nucleic acid) containing a novel pyrene LNA nucleotide monomer

被引：12

作者：

Babu, BR ^{[1
]}

Wengel, J ^{[1
]}

机构：

[1] Univ So Denmark, Dept Chem, DK-5230 Odense M, Denmark

来源：

CHEMICAL COMMUNICATIONS | 2001年 / 20期

关键词：

D O I：

10.1039/b106065c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel pyrene LNA nucleotide monomer is shown to mediate universal hybridization when incorporated into a DNA strand or a 2'-OMe-RNA/LNA chimeric strand. For the latter, high-affinity universal hybridization without compromising the base-pairing selectivity of bases neighbouring the universal pyrene LNA nucleotide monomer is documented.

引用

页码：2114 / 2115

页数：2

共 18 条

[1] BABU BR, UNPUB
[2] Universal bases for hybridization, replication and chain termination
Berger, M
Wu, YQ
Ogawa, AK
McMinn, DL
Schultz, PG
Romesberg, FE
[J]. NUCLEIC ACIDS RESEARCH, 2000, 28 (15) : 2911 - 2914
[3] Kool ET, 2000, ANGEW CHEM INT EDIT, V39, P990, DOI 10.1002/(SICI)1521-3773(20000317)39:6<990::AID-ANIE990>3.0.CO
[4] 2-0
[5] LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition
Koshkin, AA
Singh, SK
Nielsen, P
Rajwanshi, VK
Kumar, R
Meldgaard, M
Olsen, CE
Wengel, J
[J]. TETRAHEDRON, 1998, 54 (14) : 3607 - 3630
[6] Investigation of restricted backbone conformations as an explanation for the exceptional thermal stabilities of duplexes involving LNA (locked nucleic acid): synthesis and evaluation of abasic LNA
Kvaerno, L
Wengel, J
[J]. CHEMICAL COMMUNICATIONS, 1999, (07) : 657 - 658
[7] 5-NITROINDOLE AS AN UNIVERSAL BASE ANALOG
LOAKES, D
BROWN, DM
[J]. NUCLEIC ACIDS RESEARCH, 1994, 22 (20) : 4039 - 4043
[8] The applications of universal DNA base analogues
Loakes, D
[J]. NUCLEIC ACIDS RESEARCH, 2001, 29 (12) : 2437 - 2447
[9] Selective and stable DNA base pairing without hydrogen bonds
Matray, TJ
Kool, ET
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (24) : 6191 - 6192
[10] A UNIVERSAL NUCLEOSIDE FOR USE AT AMBIGUOUS SITES IN DNA PRIMERS
NICHOLS, R
ANDREWS, PC
ZHANG, P
BERGSTROM, DE
[J]. NATURE, 1994, 369 (6480) : 492 - 493

← 1 2 →