Munc18-1 promotes large dense-core vesicle docking

被引：293

作者：

Voets, T

Toonen, RF

Brian, EC

de Wit, H

Moser, T

Rettig, J

Südhof, TC

Neher, E

Verhage, M

机构：

[1] Max Planck Inst Biophys Chem, Dept Membrane Biophys, D-37077 Gottingen, Germany

[2] Univ Gottingen, Sch Med, Dept Otolaryngol, D-37073 Gottingen, Germany

[3] Univ Utrecht, Med Ctr, Mol Neurosci Rudolf Magnus Inst, NL-3584 CG Utrecht, Netherlands

[4] Univ Texas, SW Med Ctr, Dept Mol Genet, Dallas, TX 75235 USA

[5] Univ Texas, SW Med Ctr, Ctr Basic Neurosci, Howard Hughes Med Inst, Dallas, TX 75235 USA

来源：

NEURON | 2001年 / 31卷 / 04期

关键词：

D O I：

10.1016/S0896-6273(01)00391-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Secretory vesicles dock at the plasma membrane before Ca2+ triggers their exocytosis. Exocytosis requires the assembly of SNARE complexes formed by the vesicle protein Synaptobrevin and the membrane proteins Syntaxin-1 and SNAP-25. We analyzed the role of Munc18-1, a cytosolic binding partner of Syntaxin-1, in large dense-core vesicle (LDCV) secretion. Calcium-dependent LDCV exocytosis was reduced 10-fold in mouse chromaffin cells lacking Munc18-1, but the kinetic properties of the remaining release, including single fusion events, were not different from controls. Concomitantly, mutant cells displayed a 10-fold reduction in morphologically docked LDCVs. Moreover, acute overexpression of Munc18-1 in bovine chromaffin cells increased the amount of releasable vesicles and accelerated vesicle supply. We conclude that Munc18-1 functions upstream of SNARE complex formation and promotes LDCV docking.

引用

页码：581 / 591

页数：11

共 58 条

[1]

[Anonymous], BIOGENIC AMINES DEV

[2] Munc13-1 acts as a priming factor for large dense-core vesicles in bovine chromaffin cells [J].