Regulation of toxin synthesis in Clostridium difficile by an alternative RNA polymerase sigma factor

被引:188
作者
Mani, N
Dupuy, B
机构
[1] Inst Pasteur, Unite Genet Mol Bacterienne, F-75724 Paris 15, France
[2] Tufts Univ, Sch Med, Dept Mol Biol & Microbiol, Boston, MA 02211 USA
关键词
D O I
10.1073/pnas.101126598
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Clostridium difficile, a causative agent of antibiotic-associated diarrhea and its potentially lethal form, pseudomembranous colitis, produces two large protein toxins that are responsible for the cellular damage associated with the disease. The level of toxin production appears to be critical for determining the severity of the disease, but the mechanism by which toxin synthesis is regulated is unknown. The product of a gene, txeR, that lies just upstream of the tox gene cluster was shown to be needed for tox gene expression in vivo and to activate promoter-specific transcription of the fox genes in vitro in conjunction with RNA polymerases from C. difficile, Bacillus subtilis, or Escherichia coli. TxeR was shown to function as an alternative sigma factor for RNA polymerase. Because homologs of TxeR regulate synthesis of toxins and a bacteriocin in other Clostridium species. TxeR appears to be a prototype for a novel mode of regulation of toxin genes.
引用
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页码:5844 / 5849
页数:6
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