CLOSTRIDIUM-PERFRINGENS ESCHERICHIA-COLI SHUTTLE VECTORS THAT CARRY SINGLE ANTIBIOTIC-RESISTANCE DETERMINANTS

被引：90

作者：

BANNAM, TL ^{[1
]}

ROOD, JI ^{[1
]}

机构：

[1] MONASH UNIV,DEPT MICROBIOL,CLAYTON,VIC 3168,AUSTRALIA

来源：

PLASMID | 1993年 / 29卷 / 03期

关键词：

D O I：

10.1006/plas.1993.1025

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two versatile Clostridium perfringens-Escherichia coli shuttle vectors were constructed. Each plasmid carried a single antibiotic resistance gene which was expressed in both organisms. The plasmid pJIR750 encoded resistance to chloramphenicol and pJIR751 encoded resistance to erythromycin. Each plasmid contained the pUC18-derived multiple cloning site and the lacZ' gene which enabled direct screening for recombinants in E. coli. These plasmids should prove invaluable for the genetic manipulation of C. perfringens. © 1993 Academic Press, Inc.

引用

页码：233 / 235

页数：3

共 7 条

[1] HYBRIDIZATION ANALYSIS OF THE CLASS-P TETRACYCLINE RESISTANCE DETERMINANT FROM THE CLOSTRIDIUM-PERFRINGENS R-PLASMID, PCW3 [J].