Multiple interactions between RNA polymerase I, TIF-IA and TAFI subunits regulate preinitiation complex assembly at the ribosomal gene promoter

被引：80

作者：

Yuan, XJ ^{[1
]}

Zhao, J ^{[1
]}

Zentgraf, H ^{[1
]}

Hoffmann-Rohrer, U ^{[1
]}

Grummt, I ^{[1
]}

机构：

[1] German Canc Res Ctr, Div Mol Biol Cell 2, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany

来源：

EMBO REPORTS | 2002年 / 3卷 / 11期

关键词：

D O I：

10.1093/embo-reports/kvf212

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In mammals, growth-dependent regulation of rRNA synthesis is brought about by the transcription initiation factor TIF-IA. TIF-IA is associated with a fraction of the TBP-containing factor TIF-IB/SL1 and the initiation-competent form of RNA polymerase I (Pol I). We investigated the mechanisms that down-regulate cellular pre-rRNA synthesis and demonstrate that nutrient starvation, density arrest and protein synthesis inhibitors inactivate TIF-IA and impair the association of TIF-IA with Pol I. Moreover, we used a panel of TIF-IA deletion mutants to map the domains that mediate the interaction of TIF-IA with Pol I and TIF-IB/SL1. We found that amino acids 512-609 interact with two subunits of Pol I, RPA43 and PAF67, whereas a short, conserved motif (LARAK, amino acids 411-415) is required for the association of TIF-IA with TAF(I)95 and TAF(I)68. The results uncover an interphase for essential protein-protein interactions that facilitate Pol I preinitiation complex formation.

引用

页码：1082 / 1087

页数：6

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