Functional modulation of ATPase of P-glycoprotein by C219, a monoclonal antibody against P-glycoprotein

P-glycoprotein functions as an ATP driven efflux pump for antitumor agents, C219 is a monoclonal antibody which recognizes regions near both ATP binding domains in each half of P-glycoprotein. In this study, we have demonstrated that C219 inhibits the ATPase activity of P-glycoprotein based on the following findings: 1) the inhibition of total ATPase activity by C219 was selective to P-glycoprotein-positive membranes; 2) the C219-sensitive fraction of ATPase correlated the expression of P-glycoprotein; and 3) modulators of P-glycoprotein ATPase, verapamil and cyclosporin A, affected the C219-sensitive fraction of ATPase. The photolabeling of P-glycoprotein with 8-azido-[alpha-P-32]ATP was inhibited by C219, suggesting that the inhibition of ATP binding by C219 reduced the activity, Since C219 interacts with P-glycoprotein ATPase, C219 might become a useful tool for studying the role of P-glycoprotein ATPase. (C) 1997 Academic Press