Differential regulation of uncoupling protein-1, -2 and -3 gene expression by sympathetic innervation in brown adipose tissue of thermoneutral or cold-exposed rats

被引：48

作者：

Denjean, F ^{[1
]}

Lachuer, J ^{[1
]}

Géloën, A ^{[1
]}

Cohen-Adad, F ^{[1
]}

Moulin, C ^{[1
]}

Barré, H ^{[1
]}

Duchamp, C ^{[1
]}

机构：

[1] Univ Lyon 1, Lab Physiol Regulat Energet Cellulaires & Mol, CNRS, UMR 5578, F-69622 Villeurbanne, France

来源：

FEBS LETTERS | 1999年 / 444卷 / 2-3期

关键词：

unilateral sympathetic denervation; semiquantitative reverse transcriptase polymerase chain reaction; cold exposure;

D O I：

10.1016/S0014-5793(99)00056-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The control of uncoupling protein-1, -2 and -3 (UCP-1, UCP-2, UCP-3) mRNA levels by sympathetic innervation in rats was investigated by specific and sensitive RT-PCR assays. In rats reared at thermoneutrality (25 degrees C), unilateral surgical sympathetic denervation of interscapular brown adipose tissue (BAT) markedly reduced the UCP-1 mRNA level (-38%) as compared with the contralateral innervated BAT pad, but was without significant effect on UCP-2 and -3 mRNA levels. Cold exposure (7 days, 4 degrees C) markedly increased UCP-1 (+180%), UCP-2 (+115%) and UCP-3 (+195%) mRNA levels in interscapular BAT. Unilateral sympathetic denervation prevented the cold-induced rise in BAT UCP-1 and UCP-2 mRNAs, but not that in BAT UCP-3 mRNA, Results were confirmed by Northern blot analysis, These data indicate a differential endocrine control of UCP-1, UCP-2 and UCP-3 gene expression in rat BAT both at thermoneutrality and during prolonged cold exposure, (C) 1999 Federation of European Biochemical Societies.

引用

页码：181 / 185

页数：5

共 23 条

[21] Seydoux J, 1978, Experientia Suppl, V32, P153
[22] UCP3: An uncoupling protein homologue expressed preferentially and abundantly in skeletal muscle and brown adipose tissue
VidalPuig, A
Solanes, G
Grujic, D
Flier, JS
Lowell, BB
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 235 (01) : 79 - 82
[23] QUANTITATION OF MESSENGER-RNA BY THE POLYMERASE CHAIN-REACTION
WANG, AM
DOYLE, MV
MARK, DF
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (24) : 9717 - 9721

← 1 2 3 →