Intracellular localization of cyclin-dependent kinase 5 (CDK5) in mouse neuron: CDK5 is located in both nucleus and cytoplasm

被引：38

作者：

Ino, H

Chiba, T

机构：

[1] Third Department of Anatomy, Chiba University School of Medicine, Chiba 260, 1-8-1 Inohana, Chuo-ku

来源：

BRAIN RESEARCH | 1996年 / 732卷 / 1-2期

关键词：

cyclin-dependent kinase; immunohistochemistry; Purkinje cell; trigeminal ganglia; nuclear protein; central nervous system; peripheral nervous system;

D O I：

10.1016/0006-8993(96)00523-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Cyclin-dependent kinase 5 (CDK5) is one of the cyclin-dependent kinases, and is expressed in mature neurons. CDK5 has been postulated to be a neurofilament or tau protein kinase, because it phosphorylates neurofilaments and tau protein (microtubule-associated protein tau) in vitro. It has been reported that CDK5 was immunohistochemically detected only in axons of neurons. We here report the immunohistochemical study of CDK5 using two distinct antibodies, one recognizing the N-terminal of CDK5 and the other the C-terminal. Immunoreactivity of CDK5 was found not only in axons, but also intensively in nuclei, though not in nucleoli, of neurons in the mouse central and peripheral nervous systems. The nuclear CDK5 possibly has a physiological function distinct from the neurofilament or tau protein kinase.

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页码：179 / 185

页数：7

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