Cellular resolution circuit mapping with temporal-focused excitation of soma targeted channelrhodopsin

被引：106

作者：

Baker, Christopher A. ^{[1
]}

Elyadat, Yishai M. ^{[2
]}

Parra, Andres ^{[2
]}

Bolton, M. McLean ^{[1
]}

机构：

[1] Max Planck Florida Inst Neurosci, Disorders Neural Circuit Funct, Jupiter, FL USA

[2] Max Planck Florida Inst Neurosci, Funct Architecture Cerebral Cortex, Jupiter, FL USA

来源：

ELIFE | 2016年 / 5卷

关键词：

NEURONAL-ACTIVITY; 2-PHOTON OPTOGENETICS; NEURAL CIRCUITS; K+ CHANNEL; LIGHT; PHOTOSTIMULATION; CONNECTIVITY; ILLUMINATION; INHIBITION; MICROSCOPY;

D O I：

10.7554/eLife.14193

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

We describe refinements in optogenetic methods for circuit mapping that enable measurements of functional synaptic connectivity with single-neuron resolution. By expanding a two-photon beam in the imaging plane using the temporal focusing method and restricting channelrhodopsin to the soma and proximal dendrites, we are able to reliably evoke action potentials in individual neurons, verify spike generation with GCaMP6s, and determine the presence or absence of synaptic connections with patch-clamp electrophysiological recording.

引用

页数：15

共 35 条

[1]

Two-photon single-cell optogenetic control of neuronal activity by sculpted light [J].

Andrasfalvy, Bertalan K. ;

Zemelman, Boris V. ;

Tang, Jianyong ;

Vaziri, Alipasha .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (26) :11981-11986

[2]

Ultrasensitive fluorescent proteins for imaging neuronal activity [J].

Chen, Tsai-Wen ;

Wardill, Trevor J. ;

Sun, Yi ;

Pulver, Stefan R. ;

Renninger, Sabine L. ;

Baohan, Amy ;

Schreiter, Eric R. ;

Kerr, Rex A. ;

Orger, Michael B. ;

Jayaraman, Vivek ;

Looger, Loren L. ;

Svoboda, Karel ;

Kim, Douglas S. .

NATURE, 2013, 499 (7458) :295-+

[3]

Simultaneous two-photon imaging and photo-stimulation with structured light illumination [J].

Dal Maschio, Marco ;

Difato, Francesco ;

Beltramo, Riccardo ;

Blau, Axel ;

Benfenati, Fabio ;

Fellin, Tommaso .

OPTICS EXPRESS, 2010, 18 (18) :18720-18731

[4]

Dense Inhibitory Connectivity in Neocortex [J].

Fino, Elodie ;

Yuste, Rafael .

NEURON, 2011, 69 (06) :1188-1203

[5]

A targeting motif involved in sodium channel clustering at the axonal initial segment [J].

Garrido, JJ ;

Giraud, P ;

Carlier, E ;

Fernandes, F ;

Moussif, A ;

Fache, MP ;

Debanne, D ;

Dargent, B .

SCIENCE, 2003, 300 (5628) :2091-2094

[6]

Channelrhodopsin-2 Localised to the Axon Initial Segment [J].

Grubb, Matthew S. ;

Burrone, Juan .

PLOS ONE, 2010, 5 (10)

[7]

Specific Sorting and Post-Golgi Trafficking of Dendritic Potassium Channels in Living Neurons [J].

Jensen, Camilla Stampe ;

Watanabe, Shoji ;

Rasmussen, Hanne Borger ;

Schmitt, Nicole ;

Olesen, Soren-Peter ;

Frost, Nicholas A. ;

Blanpied, Thomas A. ;

Misonou, Hiroaki .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (15) :10566-10581

[8]

Klapoetke NC, 2014, NAT METHODS, V11, P338, DOI [10.1038/NMETH.2836, 10.1038/nmeth.2836]

[9]

Myosin-dependent targeting of transmembrane proteins to neuronal dendrites [J].

Lewis, Tommy L., Jr. ;

Mao, Tianyi ;

Svoboda, Karel ;

Arnold, Don B. .

NATURE NEUROSCIENCE, 2009, 12 (05) :568-576

[10]

A novel targeting signal for proximal clustering of the KV2.1 K+ channel in hippocampal neurons [J].

Lim, ST ;

Antonucci, DE ;

Scannevin, RH ;

Trimmer, JS .

NEURON, 2000, 25 (02) :385-397

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