Preparation of gold-labeled antibody probe and its use in immunochromatography assay for detection of aflatoxin B1

被引：156

作者：

Sun, XL

Zhao, XL

Tang, J

Zhou, J

Chu, FS

机构：

[1] So Yangtze Univ, Sch Food Sci, Wuxi 214036, Jiangsu, Peoples R China

[2] Gene Co Shanghai, Shanghai 210005, Peoples R China

[3] Univ Wisconsin, Food Res Inst, Madison, WI 53706 USA

[4] Univ Wisconsin, Dept Food Microbiol & Toxicol, Madison, WI 53706 USA

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2005年 / 99卷 / 02期

关键词：

colloidal gold; probe; conjugate; aflatoxin B1; immunochromatography; detection;

D O I：

10.1016/j.ijfoodmicro.2004.07.021

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Preparation of an antibody-colloidal gold probe (conjugate) specific to aflatoxin B1 (AFB1) and its use in developing a rapid AFB1 diagnostic method was presented in this paper. Monodispersional nanogold colloid was synthesized and preparation of nanogold-labeled polyclonal antibody probe to aflatoxin B1 under friendly and optimal condition. Combination of antibody with nanogold particles was also characterized by U-V-visible (UV-vis) light absorption spectra, transmission electron microscopy (TEM), fluorescence spectroscopy, titers, cross reactivity and stability measurements. Furthermore, nanogold-labeled probe was used to develop an immunochromatographic (IC) method for aflatoxin B1 analysis. With this method, analysis could be completed in less than 10 min. Detection time was reduced 6-10 times comparative with ELISA. With visual observation, lower test limit was found to be around 2.5 ng/ml aflatoxin B1 standard solution, which was increased to two times of ELISA. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：185 / 194

页数：10

共 22 条

[11]

2-P

[12] Studies on the formation of bioconjugates of Endoglucanase with colloidal gold [J].