Spatiotemporal expression of CD15 in the developing chick retina

The spatiotemporal distribution pattern of the carbohydrate epitope CD15 was examined in the developing chick retina. CD15 expression appeared for the first time at E13 in the INL and GCL. The developmental profile of the INL, from E13 to E16, showed increasing numbers of stratified amacrine cells, whereas diffuse amacrine subtypes appeared later, beginning at E15. Smaller populations of bipolar cells were seen at E17. Three types of CD15-positive ganglion cells could be differentiated by E15. A gradient in the appearance of identified immunoreactive amacrine cells extended from the dorsotemporal to the ventral and nasal retina. The adult-like pattern of CD15 expression did not become established until E19. Adult-like densities of immunoreactive cells were reached toward the end of the embryonic period between E18 in the dorsotemporal and ventral retina, and E19 in the dorsonasal retina. In the adult-like retina, labelled cells became particularly numerous at its greatest circumference, and were most densely packed in the dorsotemporal retinal quadrant. From E16 to P5, three membrane-bound, CD15-positive glycoproteins of 20, 32 and 34 kDa were identified by Western blots. The time course in the appearance of the membrane-associated CD15 recognition molecule on differentiating amacrine, bipolar and ganglion cells is correlated to the establishment of synaptic contacts.