A new derivatization procedure for the determination of cephalexin with 1,2-naphthoquinone 4-sulphonate in pharmaceutical and urine samples using solid-phase extraction cartridges and UV-visible detection

被引：21

作者：

Gallo-Martinez, L ^{[1
]}

Sevillano-Cabeza, A ^{[1
]}

Campins-Falcó, P ^{[1
]}

Bosch-Reig, F ^{[1
]}

机构：

[1] Univ Valencia, Fac Quim, Dept Quim Analit, E-46100 Valencia, Spain

来源：

ANALYTICA CHIMICA ACTA | 1998年 / 370卷 / 2-3期

关键词：

cephalexin; derivatization; solid-phase extraction; UV-visible detection; pharmaceuticals; urine; H-point standard additions method; generalized H-point standard additions method;

D O I：

10.1016/S0003-2670(98)00276-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The present report shows how to derivatize cephalexin with I,2-naphthoquinone-4-sulphonate (NQS) into solid-phase extraction cartridges (Clx) using UV-visible detection. Optimum conditions for this new procedure are: hydrogen carbonate/carbonate buffer pH=10.5, 5 min reaction time at 25 degrees C and NQS concentration of 7.1 x 10(-3) mol l(-1). The accuracy and the precision of the method were tested. The procedure was used to measure cephalexin in pharmaceutical and urine samples. The results obtained were contrasted with those reported by UV-spectrophotometric and HPLC methods for pharmaceutical samples and with HPLC method for urine samples. The H-point standard additions method was used to measure cephalexin in pharmaceutical samples, and the generalized H-point standard additions method was used to measure cephalexin in urine samples. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：115 / 123

页数：9

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