Dual-genic hybridization sensor employing electrochemical impedance spectroscopy

A dual-genic impedimetric sensor for DNA hybridization detection is proposed for the first time. Two different single-stranded DNA probes (P-1 = d(pA)(20) and P-2 = d(pG)(20)) were immobilized onto the graphite-epoxy electrode surface and impedimetric measurements were performed. Hybridization experiments were carried out employing complementary targets (P-1(c) = d(pT)(20) or P-2(c)= d(PC)(20)) alone, or combined (P-1(c)+ P-2(c)) as well as noncomplementary DNA sequences. As from the direct observation of impedance spectra it was not possible to resolve the binary gene mixture, the use of artificial neural networks (ANN) was proposed for extracting significant information to get desired results.