Base-pairing between untranslated regions facilitates translation of uncapped, nonpolyadenylated viral RNA

被引：168

作者：

Guo, L ^{[1
]}

Allen, EM ^{[1
]}

Miller, WA ^{[1
]}

机构：

[1] Iowa State Univ, Dept Plant Pathol, Ames, IA 50011 USA

来源：

MOLECULAR CELL | 2001年 / 7卷 / 05期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S1097-2765(01)00252-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Translationally competent mRNAs form a closed loop via interaction of initiation factors with the 5 ' cap and poly(A) tail. However, many viral mRNAs lack a cap and/or a poly(A) tail. We show that an uncapped, nonpolyadenylated plant viral mRNA forms a closed loop by direct base-pairing (kissing) of a stem loop in the 3 ' untranslated region (UTR) with a stem loop in the 5 ' UTR. This allows a sequence in the 3 ' UTR to confer translation initiation at the 5 ' -proximal AUG. This base-pairing is also required for replication. Unlike other cap-independent translation mechanisms, the ribosome enters at the 5 ' end of the mRNA. This remarkably long-distance base-pairing reveals a novel mechanism of cap-independent translation and means by which mRNA UTRs can communicate.

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收藏

页码：1103 / 1109

页数：7

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