Protective Effects of Extracts and Flavonoids Isolated from Scutia buxifolia Reissek against Chromosome Damage in Human Lymphocytes Exposed to Hydrogen Peroxide

被引：41

作者：

Boligon, Aline Augusti ^{[1
]}

Sagrillo, Michele Rorato ^{[2
,3
]}

Machado, Luiz Filipe ^{[3
]}

de Souza Filho, Olmiro ^{[3
]}

Machado, Michel Mansur ^{[4
]}

Manica da Cruz, Ivana Beatrice ^{[3
]}

Athayde, Margareth Linde ^{[1
]}

机构：

[1] Univ Fed Santa Maria, Program Postgrad Pharmaceut Sci, BR-97105900 Santa Maria, RS, Brazil

[2] Franciscan Univ Ctr UNIFRA, BR-97010032 Santa Maria, RS, Brazil

[3] Univ Fed Santa Maria, Program Postgrad Pharmacol, BR-97105900 Santa Maria, RS, Brazil

[4] Fed Univ Pampa UNIPAMPA, BR-97500970 Uruguaiana, Brazil

来源：

MOLECULES | 2012年 / 17卷 / 05期

关键词：

Scutia buxifolia; flavonols; lymphocyte culture; chromosomes damage; HPLC/DAD; ANTIOXIDANT; FRACTIONS; QUERCETIN; ASSAY;

D O I：

10.3390/molecules17055757

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Flavonoids are claimed to protect against cardiovascular disease, certain forms of cancer and ageing, possibly by preventing initial DNA damage. Therefore, we investigated the protective effects of crude extract, ethyl acetate fraction and flavonoids (quercetin, quercitrin, isoquercitrin and rutin) isolated from the leaves from Scutia buxifolia against chromosome damage induced by H2O2 in human lymphocytes by analyzing cellular growth rate, cell viability, mitotic index and chromosomal instability. We found a differential response among the compounds tested, with the ethyl acetate fraction being more effective than the crude extract, a difference perhaps related to the presence of the antioxidants identified and quantified by HPLC/DAD. In general, quercetin, isoquercitrin and rutin recovered the mitotic index and chromosomal instability more than quercitrin after treatment with hydrogen peroxide.

引用

页码：5757 / 5769

页数：13

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