A pollen allergen-encoding gene is expressed in wheat ovaries

被引：3

作者：

Balzer, HJ ^{[1
]}

Borisiuk, L ^{[1
]}

Meyer, HM ^{[1
]}

Matzk, F ^{[1
]}

Baumlein, H ^{[1
]}

机构：

[1] INST PFLANZENGENET & KULTURPFLANZENFORSCH,ABT MOL GENET,D-06466 GATERSLEBEN,GERMANY

来源：

PLANT MOLECULAR BIOLOGY | 1996年 / 32卷 / 03期

关键词：

male sterility; ovary; parthenogenetic lines; pollen allergen; subtractive hybridization; wheat;

D O I：

10.1007/BF00019095

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To isolate genes specifically expressed at the initiation of plant embryo development we have applied a sensitive subtractive hybridization technique for three isogenic wheat lines of the so-called 'Salmon system' with either zygotic or autonomous embryo development. Here we present a gene sequence showing a high homology to grass pollen allergens of type II/III thought to be expressed in pollen tissue only. Surprisingly, the pollen allergen-like sequence, designated Tri a III, is also expressed in gynoecia of the sexual, male fertile wheat line '(aestivum)-Salmon', whereas the two parthenogenetic and male sterile wheat lines '(caudata)-Salmon' and '(kotschyi)-Salmon' completely lack any Tri a III transcript. Our data suggest a positive correlation between the expression of this clone and the manifestation of male fertility. Northern and in situ hybridization analysis revealed that, in addition to its presence in pollen, Tri a III is expressed in the parenchymatous tissue of '(aestivum)-Salmon' ovaries exclusively at the day of anthesis. This precise temporal and spatial expression pattern suggests a more general function of the pollen allergen-like sequence Tri a LU not limited to the exhibition of allergens in pollen grains.

引用

页码：435 / 445

页数：11

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