The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions

被引：297

作者：

Denny, Paul ^{[1
]}

Hagen, Fred K. ^{[2
]}

Hardt, Markus ^{[3
]}

Liao, Lujian ^{[4
]}

Yan, Weihong ^{[5
]}

Arellanno, Martha ^{[9
]}

Bassilian, Sara

Bedi, Gurrinder S. ^{[2
]}

Boontheung, Pinmannee ^{[5
]}

Cociorva, Daniel ^{[4
]}

Delahunty, Claire M. ^{[4
]}

Denny, Trish ^{[1
]}

Dunsmore, Jason

Faull, Kym F. ^{[10
]}

Gilligan, Joyce ^{[1
]}

Gonzalez-Begne, Mireya ^{[2
]}

Halgand, Frederic ^{[10
]}

Hall, Steven C. ^{[3
,8
]}

Han, Xuemei ^{[4
]}

Henson, Bradley ^{[9
]}

Hewel, Johannes

Hu, Shen

Jeffrey, Sherry ^{[9
]}

Jiang, Jiang

Loo, Joseph A. ^{[5
,8
,11
]}

Loo, Rachel R. Ogorzallek ^{[8
]}

Malamud, Daniel ^{[6
]}

Melvin, James E. ^{[2
]}

Miroshnychenko, Olga ^{[3
]}

Navazesh, Mahvash ^{[1
]}

Niles, Richard ^{[3
]}

Park, Sung Kyu

Prakobphol, Akraporn ^{[3
]}

Ramachandran, Prasanna ^{[5
]}

Richert, Megan ^{[9
]}

Robinson, Sarah ^{[3
]}

Sondej, Melissa ^{[5
]}

Souda, Puneet ^{[10
]}

Sullivan, Mark A. ^{[2
]}

Takashima, Jona ^{[1
]}

Than, Shawn ^{[9
]}

Wang, Jianghua ^{[9
]}

Whitelegge, Julian P. ^{[10
]}

Witkowska, H. Ewa ^{[3
,8
]}

Wolinsky, Lawrence ^{[9
]}

Xie, Yongming ^{[5
]}

Xu, Tao ^{[4
]}

Yu, Weixia ^{[9
]}

Ytterberg, Jimmy ^{[5
]}

Wong, David T. ^{[9
,11
,12
]}

机构：

[1] Univ So Calif, Sch Dent, Los Angeles, CA 90089 USA

[2] Univ Rochester, Med Ctr, Ctr Oral Biol, Rochester, NY 14642 USA

[3] Univ Calif San Francisco, Dept Cell & Tissue Biol, San Francisco, CA 94143 USA

[4] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[5] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

[6] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA

[7] Univ Calif Berkeley, Lawrence Berkeley Lab, Berkeley, CA 94720 USA

[8] Univ Calif Los Angeles, David Geffen Sch Med, Los Angeles, CA 90095 USA

[9] Univ Calif Los Angeles, Sch Dent, Los Angeles, CA 90095 USA

[10] Univ Calif Los Angeles, Semel Inst Neurosci & Human Behav, Los Angeles, CA 90095 USA

[11] Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Los Angeles, CA 90095 USA

[12] Univ Calif Los Angeles, Div Otolaryngol Head & Neck Surg, Los Angeles, CA 90095 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2008年 / 7卷 / 05期

关键词：

human saliva; parotid; submandibular; sublingual; ductal secretion; proteomics; mass spectrometry;

D O I：

10.1021/pr700764j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Saliva is a body fluid with important functions in oral and general health. A consortium of three research groups catalogued the proteins in human saliva collected as the ductal secretions: 1166 identifications-914 in parotid and 917 in submandibular/sublingual saliva-were made. The results showed that a high proportion of proteins that are found in plasma and/or tears are also present in saliva along with unique components. The proteins identified are involved in numerous molecular processes ranging from structural functions to enzymatic/catalytic activities. As expected, the majority mapped to the extracellular and secretory compartments. An immunoblot approach was used to validate the presence in saliva of a subset of the proteins identified by mass spectrometric approaches. These experiments focused on novel constituents and proteins for which the peptide evidence was relatively weak. Ultimately, information derived from the work reported here and related published studies can be used to translate blood-based clinical laboratory tests into a format that utilizes saliva. Additionally, a catalogue of the salivary proteome of healthy individuals allows future analyses of salivary samples from individuals with oral and systemic diseases, with the goal of identifying biomarkers with diagnostic and/or prognostic value for these conditions; another possibility is the discovery of therapeutic targets.

引用

页码：1994 / 2006

页数：13

共 103 条

[1] The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins [J].