A re-investigation of the thio effect at the hammerhead cleavage site

被引：86

作者：

Scott, EC ^{[1
]}

Uhlenbeck, OC ^{[1
]}

机构：

[1] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 02期

关键词：

D O I：

10.1093/nar/27.2.479

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of introducing a phosphorothioate at the hammerhead cleavage site was investigated using a kinetically well-characterized hammerhead, In buffers containing Mg ion, the R(P)-phosphorothioate isomer cleaved 2000- to 80 000-fold slower than the Sp isomer or the unmodified RNA substrate. Addition of low concentrations of several thiophilic metal ions, especially Cd(2+), to these reactions is sufficient to fully restore the cleavage rate of the R(P) substrate without affecting cleavage rate of the all-oxygen or S(P) substrate, Thus, a model proposing coordination of a divalent metal ion to the pro-R oxygen at the hammerhead cleavage site appears justified.

引用

页码：479 / 484

页数：6

共 38 条

[21] Rescue of abasic hammerhead ribozymes by exogenous addition of specific bases [J].

Peracchi, A ;

Beigelman, L ;

Usman, N ;

Herschlag, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (21) :11522-11527

[22] Involvement of a specific metal ion in the transition of the hammerhead ribozyme to its catalytic conformation [J].

Peracchi, A ;

Beigelman, L ;

Scott, EC ;

Uhlenbeck, OC ;

Herschlag, D .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (43) :26822-26826

[23] METAL-ION CATALYSIS IN THE TETRAHYMENA RIBOZYME REACTION [J].

PICCIRILLI, JA ;

VYLE, JS ;

CARUTHERS, MH ;

CECH, TR .

NATURE, 1993, 361 (6407) :85-88

[24] 3-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME [J].

PLEY, HW ;

FLAHERTY, KM ;

MCKAY, DB .

NATURE, 1994, 372 (6501) :68-74

[25] Observations on catalysis by hammerhead ribozymes are consistent with a two-divalent-metal-ion mechanism [J].

Pontius, BW ;

Lott, WB ;

vonHippel, PH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2290-2294

[26] AUTOLYTIC PROCESSING OF DIMERIC PLANT-VIRUS SATELLITE RNA [J].

PRODY, GA ;

BAKOS, JT ;

BUZAYAN, JM ;

SCHNEIDER, IR ;

BRUENING, G .

SCIENCE, 1986, 231 (4745) :1577-1580

[27]

SAENGER W, 1988, SPRINGER ADV TEXTS C

[28] Capturing the structure of a catalytic RNA intermediate: The hammerhead ribozyme [J].

Scott, WG ;

Murray, JB ;

Arnold, JRP ;

Stoddard, BL ;

Klug, A .

SCIENCE, 1996, 274 (5295) :2065-2069

[29] THE CRYSTAL-STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME - A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE [J].

SCOTT, WG ;

FINCH, JT ;

KLUG, A .

CELL, 1995, 81 (07) :991-1002

[30] SITE-DIRECTED MUTAGENESIS OF HISTIDINE-13 AND HISTIDINE-114 OF HUMAN ANGIOGENIN - ALANINE DERIVATIVES INHIBIT ANGIOGENIN-INDUCED ANGIOGENESIS [J].

SHAPIRO, R ;

VALLEE, BL .

BIOCHEMISTRY, 1989, 28 (18) :7401-7408

← 1 2 3 4 →