High throughput assay for the determination of lumefantrine in plasma

被引：44

作者：

Annerberg, A

Singtoroj, T

Tipmanee, P

White, NJ

Day, NPJ

Lindegårdh, N

机构：

[1] Mahidol Univ, Fac Trop Med, Wellcome Unit, Bangkok 10400, Thailand

[2] Univ Oxford, Ctr Trop Med, Nuffield Dept Clin Med, Oxford, England

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 822卷 / 1-2期

基金：

英国惠康基金;

关键词：

antimalarial; high throughput; liquid chromatography; lumefantrine; solid phase extraction; 96-well;

D O I：

10.1016/j.jchromb.2005.06.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high throughput bioanalytical assay for the determination of lumefantrine in plasma has been developed and validated extensively. The within-day precisions for lumefantrine were 5.2, 3.5 and 2.5% at 200, 2000 and 15000 ng/mL, respectively. The between-day precisions were 4.0, 2.8 and 3.1 % at 200, 2000 and 15000 ng/mL, respectively. The lower limits of quantification (LLOQ) and the limits of detection (LOD) were 25 and 10 ng/mL, respectively using 0.250 mL plasma. The average recovery of lumefantrine was 85% and independent upon concentration. The use of 96-well plate format and short chromatographic run has increased the daily sample throughput four times. The assay is particularly suitable for large therapeutic drug monitoring studies using day 7 sampling. (c) 2005 Elsevier B.V. All rights reserved.

引用

收藏

页码：330 / 333

页数：4

相关论文

共 13 条

[1] Binding of artemether and lumefantrine to plasma proteins and erythrocytes [J].

Colussi, D ;

Parisot, C ;

Legay, F ;

Lefèvre, G .

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 1999, 9 (01) :9-16

[2]

*DEP HHS, 2001, GUID IND BIOAN METH

[3] Pharmacokinetics and pharmacodynamics of lumefantrine (benflumetol) in acute falciparum malaria [J].

Ezzet, F ;

van Vugt, M ;

Nosten, F ;

Looareesuwan, S ;

White, NJ .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (03) :697-704

[4] Efficacy and safety of artemether-lumefantrine (Coartem®) tablets (six-dose regimen) in African infants and children with acute) uncomplicated falcipaurum malaria [J].

Falade, C ;

Makanga, M ;

Premji, Z ;

Ortmann, CE ;

Stockmeyer, M ;

de Palacios, PI .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2005, 99 (06) :459-467

[5] Efficacy and safety of CGP 56697 (artemether and benflumetol) compared with chloroquine to treat acute falciparum malaria in Tanzanian children aged 1-5 years [J].

Hatz, C ;

Abdulla, S ;

Mull, R ;

Schellenberg, D ;

Gathmann, I ;

Kibatala, P ;

Beck, HP ;

Tanner, M ;

Royce, C .

TROPICAL MEDICINE & INTERNATIONAL HEALTH, 1998, 3 (06) :498-504

[6] Development and validation of a bioanalytical method using automated solid-phase extraction and LC-UV for the simultaneous determination of lumefantrine and its desbutyl metabolite in plasma [J].

Lindegårdh, N ;

Annerberg, A ;

Blessborn, D ;

Bergqvist, Y ;

Day, N ;

White, NJ .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2005, 37 (05) :1081-1088

[7] Determination of a new antimalarial drug, benflumetol, in blood plasma by high-performance liquid chromatography [J].

Mansor, SM ;

Navaratnam, V ;

Yahaya, N ;

Nair, NK ;

Wernsdorfer, WH ;

Degen, PH .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1996, 682 (02) :321-325

[8] Randomized comparison of chloroquine plus sulfadoxine-pyrimethamine versus artesunate plus mefloquine versus artemether-lumefantrine in the treatment of uncomplicated falciparum malaria in the Lao People's Democratic Republic [J].

Mayxay, M ;

Khanthavong, M ;

Lindegårdh, N ;

Keola, S ;

Barends, M ;

Pongvongsa, T ;

Yapom, R ;

Annerberg, A ;

Phompida, S ;

Phetsouvanh, R ;

White, NJ ;

Newton, PN .

CLINICAL INFECTIOUS DISEASES, 2004, 39 (08) :1139-1147

[9] Therapeutic efficacy of artemether-lumefantrine and artesunate-mefloquine for treatment of uncomplicated Plasmodium falciparum malaria in Luang Namtha Province, Lao People's Democratic Republic [J].

Stohrer, JM ;

Dittrich, S ;

Thongpaseuth, V ;

Vanisaveth, V ;

Phetsouvanh, R ;

Phompida, S ;

Monti, F ;

Christophel, EM ;

Lindegardh, N ;

Annerberg, A ;

Jelinek, T .

TROPICAL MEDICINE & INTERNATIONAL HEALTH, 2004, 9 (11) :1175-1183

[10] Artemether-lumefantrine for the treatment of multidrug-resistant falciparum malaria [J].

van Vugt, M ;

Looareesuwan, S ;

Wilairatana, P ;

McGready, R ;

Villegas, L ;

Gathmann, I ;

Mull, R ;

Brockman, A ;

White, NJ ;

Nosten, F .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2000, 94 (05) :545-548