Effects of dynamic exercise on mean blood velocity and muscle interstitial metabolite responses in humans

被引:54
作者
Lott, MEJ
Hogeman, CS
Vickery, L
Kunselman, AR
Sinoway, LI
MacLean, DA
机构
[1] Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Med,Div Cardiol,MC H047, Hershey, PA 17033 USA
[2] Penn State Univ, Milton S Hershey Med Ctr, Coll Med, Dept Hlth Evaluat Sci, Hershey, PA 17033 USA
[3] Lebanon Vet Affairs Med Ctr, Lebanon, PA 17042 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2001年 / 281卷 / 04期
关键词
vasodilation; blood flow; microdialysis;
D O I
10.1152/ajpheart.2001.281.4.H1734
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We examined the effects of dynamic one-legged knee extension exercise on mean blood velocity (MBV) and muscle interstitial metabolite concentrations in healthy young subjects (n = 7). Femoral MBV (Doppler), mean arterial pressure (MAP) and muscle interstitial metabolite (adenosine, lactate, phosphate, K+, pH, and H+; by microdialysis) concentrations were measured during 5 min of exercise at 30 and 60% of maximal work capacity (W-max). MAP increased (P < 0.05) to a similar extent during the two exercise bouts, whereas the increase in MBV was greater (P < 0.05) during exercise at 60% (77.00 +/- 6.77 cm/s) compared with 30% W-max (43.71 +/- 3.71 cm/s). The increase in interstitial adenosine from rest to exercise was greater (P < 0.05) during the 60% (0.80 +/- 0.10 <mu>M) compared with the 30% W-max bout (0.57 +/- 0.10 muM). During exercise at 60% W-max interstitial K+ rose at a greater rate than during exercise at 30% W-max (P < 0.05). However, pH increased (H+ decreased) at similar rates for the two exercise intensities. During exercise, interstitial lactate and phosphate increased (P < 0.05) with no difference observed between the two intensities. After 5 min of recovery, MBV decreased to baseline levels after exercise at 30% Wm,, (4.12 +/- 1.10 cm/s), whereas MBV remained above baseline levels after exercise at 60% W-max (Delta 19.46 +/- 2.61 cm/s; P < 0.05). MAP and interstitial adenosine, K+, pH, and H+ returned toward baseline levels. However, interstitial lactate and phosphate continued to increase during the recovery period. Thus an increase in exercise intensity resulted in concomitant changes in MBV and muscle interstitial adenosine and K+, whereas similar changes were not observed for MAP or muscle interstitial pH, lactate, or phosphate. These data suggest that K+ and/or adenosine may play an active role in the regulation of skeletal muscle blood flow during exercise.
引用
收藏
页码:H1734 / H1741
页数:8
相关论文
共 39 条
[11]   Interstitial K+ in human skeletal muscle during and after dynamic graded exercise determined by microdialysis [J].
Juel, C ;
Pilegaard, H ;
Nielsen, JJ ;
Bangsbo, J .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2000, 278 (02) :R400-R406
[12]  
Laughlin MH., 1996, Handbook of Physiology Exercise: Regulation and Integration of Multiple Systems, P705
[13]   A MICRODIALYSIS METHOD ALLOWING CHARACTERIZATION OF INTERCELLULAR WATER SPACE IN HUMANS [J].
LONNROTH, P ;
JANSSON, PA ;
SMITH, U .
AMERICAN JOURNAL OF PHYSIOLOGY, 1987, 253 (02) :E228-E231
[14]   Effects of hindlimb contraction on pressor and muscle interstitial metabolite responses in the cat [J].
MacLean, D ;
LaNoue, KF ;
Gray, KS ;
Sinoway, LI .
JOURNAL OF APPLIED PHYSIOLOGY, 1998, 85 (04) :1583-1592
[15]   Femoral arterial injection of adenosine in humans elevates MSNA via central but not peripheral mechanisms [J].
MacLean, DA ;
Saltin, B ;
Radegran, G ;
Sinoway, L .
JOURNAL OF APPLIED PHYSIOLOGY, 1997, 83 (04) :1045-1053
[16]   Muscle interstitial glucose and lactate levels during dynamic exercise in humans determined by microdialysis [J].
MacLean, DA ;
Bangsbo, J ;
Saltin, B .
JOURNAL OF APPLIED PHYSIOLOGY, 1999, 87 (04) :1483-1490
[17]   Interstitial pH, K+, lactate, and phosphate determined with MSNA during exercise in humans [J].
MacLean, DA ;
Imadojemu, VA ;
Sinoway, LI .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2000, 278 (03) :R563-R571
[18]  
MACLEAN DA, 1995, J PHYSL LOND P, V491, P126
[19]   PHYSIOLOGICAL IMPORTANCE OF ATP RELEASED FROM NERVE-TERMINALS AND ITS DEGRADATION TO ADENOSINE IN HUMANS [J].
PELLEG, A ;
BURNSTOCK, G .
CIRCULATION, 1990, 82 (06) :2269-2272
[20]   Microdialysis ethanol removal reflects probe recovery rather than local blood flow in skeletal muscle [J].
Rådegran, G ;
Pilegaard, H ;
Nielsen, JJ ;
Bangsbo, J .
JOURNAL OF APPLIED PHYSIOLOGY, 1998, 85 (02) :751-757