The effect of potentially toxic elements and sewage sludge on the activity of regulatory enzyme glutamate kinase

被引：22

作者：

Pavlikova, D. ^{[1
]}

Pavlik, M. ^{[2
]}

Staszkova, L. ^{[1
]}

Tlustos, P. ^{[1
]}

Szakova, J. ^{[1
]}

Balik, J. ^{[1
]}

机构：

[1] Czech Univ Life Sci Prague, Fac Agrobiol Food & Nat Resources, Prague 16521 6, Czech Republic

[2] Acad Sci Czech Republ, Inst Expt Bot, Prague, Czech Republic

来源：

PLANT SOIL AND ENVIRONMENT | 2007年 / 53卷 / 05期

关键词：

proline regulation; toxic elements; plant stress metabolism; chronic stress;

D O I：

10.17221/2204-PSE

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The glutamate kinase activity was investigated as a plant stress response to Cd, Zn, As or sewage sludge application to soil in the pot and field experiments with spinach (Spinacia oleracea L.). Allosteric regulation of glutamate kinase activity by free proline creates a possibility for an increase in glutamic acid content due to the synthesis of glutathione and phytochelatines in plant cells. For this reason the high rates of As, Cd and Zn applied into soil strongly decreased the glutamate kinase activity. Allosteric regulation of the glutamate kinase activity did not inhibit the synthesis of proline and hydroxyproline under stress condition caused by organic pollutants after application of sewage sludge. Formed proline was bound to stress proteins and therefore glutamate kinase activity was not inhibited.

引用

页码：201 / 206

页数：6

共 26 条

[1]

Balík J, 2000, ROST VYROBA, V46, P273

[2]

Baran S, 2003, POL J ENVIRON STUD, V12, P523

[3] Bacteroid proline catabolism affects N2 fixation rate of drought-stressed soybeans [J].

Curtis, J ;

Shearer, G ;

Kohl, DH .

PLANT PHYSIOLOGY, 2004, 136 (02) :3313-3318

[4] Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance [J].

Demiral, T ;

Türkan, I .

ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2005, 53 (03) :247-257

[5] Mitochondrial transport in proline catabolism in plants: the existence of two separate translocators in mitochondria isolated from durum wheat seedlings [J].

Di Martino, C ;

Pizzuto, R ;

Pallotta, ML ;

De Santis, A ;

Passarella, S .

PLANTA, 2006, 223 (06) :1123-1133

[6] Cloning of a polycistronic cDNA from tomato encoding gamma-glutamyl kinase and gamma-glutamyl phosphate reductase [J].

GarciaRios, M ;

Fujita, T ;

LaRosa, PC ;

Locy, RD ;

Clithero, JM ;

Bressan, RA ;

Csonka, LN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (15) :8249-8254

[7] Interactive effects of sodium chloride and calcium chloride on the accumulation of proline and glycinebetaine in peanut (Arachis hypogaea L.) [J].

Girija, C ;

Smith, BN ;

Swamy, PM .

ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2002, 47 (01) :1-10

[8] A BIFUNCTIONAL ENZYME (DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHETASE) CATALYZES THE 1ST 2 STEPS IN PROLINE BIOSYNTHESIS IN PLANTS [J].

HU, CAA ;

DELAUNEY, AJ ;

VERMA, DPS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (19) :9354-9358

[9] Impact of soluble sugar concentrations on the acquisition of freezing tolerance in accessions of Arabidopsis thaliana with contrasting cold adaptation -: evidence for a role of raffinose in cold acclimation [J].

Klotke, J ;

Kopka, J ;

Gatzke, N ;

Heyer, AG .

PLANT CELL AND ENVIRONMENT, 2004, 27 (11) :1395-1404

[10]

Matysik J, 2002, CURR SCI INDIA, V82, P525

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