The effect of potentially toxic elements and sewage sludge on the activity of regulatory enzyme glutamate kinase

被引：22

作者：

Pavlikova, D. ^{[1
]}

Pavlik, M. ^{[2
]}

Staszkova, L. ^{[1
]}

Tlustos, P. ^{[1
]}

Szakova, J. ^{[1
]}

Balik, J. ^{[1
]}

机构：

[1] Czech Univ Life Sci Prague, Fac Agrobiol Food & Nat Resources, Prague 16521 6, Czech Republic

[2] Acad Sci Czech Republ, Inst Expt Bot, Prague, Czech Republic

来源：

PLANT SOIL AND ENVIRONMENT | 2007年 / 53卷 / 05期

关键词：

proline regulation; toxic elements; plant stress metabolism; chronic stress;

D O I：

10.17221/2204-PSE

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The glutamate kinase activity was investigated as a plant stress response to Cd, Zn, As or sewage sludge application to soil in the pot and field experiments with spinach (Spinacia oleracea L.). Allosteric regulation of glutamate kinase activity by free proline creates a possibility for an increase in glutamic acid content due to the synthesis of glutathione and phytochelatines in plant cells. For this reason the high rates of As, Cd and Zn applied into soil strongly decreased the glutamate kinase activity. Allosteric regulation of the glutamate kinase activity did not inhibit the synthesis of proline and hydroxyproline under stress condition caused by organic pollutants after application of sewage sludge. Formed proline was bound to stress proteins and therefore glutamate kinase activity was not inhibited.

引用

页码：201 / 206

页数：6

共 26 条

[11] CZECHOSLOVAKIAN BIOLOGICAL CERTIFIED REFERENCE MATERIALS AND THEIR USE IN THE ANALYTICAL QUALITY ASSURANCE SYSTEM IN A TRACE-ELEMENT LABORATORY [J].

MIHOLOVA, D ;

MADER, P ;

SZAKOVA, J ;

SLAMOVA, A ;

SVATOS, Z .

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1993, 345 (2-4) :256-260

[12] Separation of organic compounds binding trace elements in seeds of Leuzea carthamoides (Willd.) DC [J].

Pavliková, D ;

Pavlík, M ;

Vasicková, S ;

Száková, J ;

Tlustos, P ;

Vokác, K ;

Balík, J .

APPLIED ORGANOMETALLIC CHEMISTRY, 2004, 18 (12) :619-625

[13] Development of a procedure for the sequential extraction of substances binding trace elements in plant biomass [J].

Pavlíkova, D ;

Pavlík, M ;

Vasicková, S ;

Száková, J ;

Vokac, K ;

Balík, J ;

Tlustos, P .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005, 381 (04) :863-872

[14] Differential antioxidative responses to water stress among five mulberry (Morus alba L.) cultivars [J].

Reddy, AR ;

Chaitanya, KV ;

Jutur, PP ;

Sumithra, K .

ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2004, 52 (01) :33-42

[15] Response to cadmium in higher plants [J].

Sanità di Toppi, L ;

Gabbrielli, R .

ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 1999, 41 (02) :105-130

[16]

Schat H, 1997, PHYSIOL PLANTARUM, V101, P477, DOI 10.1111/j.1399-3054.1997.tb01026.x

[17] Plant responses to abiotic stresses:: heavy metal-induced oxidative stress and protection by mycorrhization [J].

Schützendübel, A ;

Polle, A .

JOURNAL OF EXPERIMENTAL BOTANY, 2002, 53 (372) :1351-1365

[18] In vitro alleviation of heavy metal-induced enzyme inhibition by proline [J].

Sharma, SS ;

Schat, H ;

Vooijs, R .

PHYTOCHEMISTRY, 1998, 49 (06) :1531-1535

[19] Hg and Cd induced changes in proline content and activities of proline biosynthesizing enzymes in Phaseolus aureus and Triticum aestivum [J].

Shaw, BP ;

Rout, NP .

BIOLOGIA PLANTARUM, 2002, 45 (02) :267-271

[20] REGULATION OF PROLINE-INHIBITABLE GLUTAMATE KINASE (EC 2.7.2.11, ATP - GAMMA-L-GLUTAMATE PHOSPHOTRANSFERASE) OF WINTER-WHEAT LEAVES BY MONO-VALENT CATIONS AND L-PROLINE [J].

STEFL, M ;

VASAKOVA, L .

COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS, 1984, 49 (11) :2698-2708

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