Low-affinity nerve-growth factor receptor (P75NTR) can serve as a receptor for rabies virus

被引:212
作者
Tuffereau, C [1 ]
Bénéjean, J
Blondel, D
Kieffer, B
Flamand, A
机构
[1] CNRS, Lab Genet Virus, F-91198 Gif Sur Yvette, France
[2] ESBS, UPR 9050 CNRS, Lab Prot & Recepteurs Membranaires, F-67400 Illkirch Graffenstaden, France
关键词
expression cloning; glycoprotein; p75NTR; rabies virus; receptor;
D O I
10.1093/emboj/17.24.7250
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A random-primed cDNA expression library constructed from the mRNA of neuroblastoma cells (NG108) was used to clone a specific rabies virus (RV) receptor. A soluble form of the RV glycoprotein (G(s)) was utilized as a ligand to detect positive cells. We identified the murine low-affinity nerve-growth factor receptor, p75NTR, BSR cells stably expressing p75NTR were able to bind G(s) and G-expressing lepidopteran cells. The ability of the RV glycoprotein to bind p75NTR was dependent on the presence of a lysine and arginine in positions 330 and 333 respectively of antigenic site III, which is known to control virus penetration into motor and sensory neurons of adult mice. P75NTR-expressing BSR cells were permissive for a non-adapted fox RV isolate (street virus) and nerve growth factor (NGF) decreased this infection. In infected cells, p75NTR associates with the RV glycoprotein and could be precipitated with anti-G monoclonal antibodies. Therefore, p75NTR is a receptor for street RV.
引用
收藏
页码:7250 / 7259
页数:10
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