End4/Sla2 is involved in establishment of a new growth zone in Schizosaccharomyces pombe

被引:40
作者
Castagnetti, S
Behrens, R
Nurse, P
机构
[1] Canc Res UK, Cell Cycle Lab, London WC2A 3PX, England
[2] Paul Ehrlich Inst, D-63225 Langen, Germany
[3] Rockefeller Univ, New York, NY 10021 USA
关键词
End4/Sla2; fission yeast; Tea1; cell growth;
D O I
10.1242/jcs.02311
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The rod-shaped Schizosaccharomyces pombe cell grows in a polarized fashion from opposing ends. Correct positioning of the growth zones is directed by the polarity marker Teal located at the cell ends where actin patches accumulate and cell growth takes place. We show that the S. pombe homologue of Saccharomyces cerevisiae SLA2, a protein involved in cortical actin organization and endocytosis, provides a link between the polarity marker and the growth machinery. In wild-type fission yeast cells, this homologue End4/Sla2 is enriched at cell ends during interphase and localizes to a medial ring at cell division, mirroring the actin localization pattern throughout the cell cycle. Proper localization relies on membrane trafficking and is independent of both the actin and microtubule cytoskeletons. End4/Sla2 is required for the establishment of new polarised growth zones, and deletion of its C-terminal talin-like domain prevents the establishment of a new growth zone after cell fission. We propose that End4/Sla2 acts downstream of the polarity marker Teal and is implicated in the recruitment of the actin cytoskeleton to bring about polarised cell growth.
引用
收藏
页码:1843 / 1850
页数:8
相关论文
共 45 条
[31]  
MORENO S, 1991, METHOD ENZYMOL, V194, P795
[32]   Requirement of an intact microtubule cytoskeleton for aggregation and inclusion body formation by a mutant huntingtin fragment [J].
Muchowski, PJ ;
Ning, K ;
D'Souza-Schorey, C ;
Fields, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (02) :727-732
[33]   Equatorial retention of the contractile actin ring by microtubules during cytokinesis [J].
Pardo, M ;
Nurse, P .
SCIENCE, 2003, 300 (5625) :1569-1574
[34]   END3 AND END4 - 2 MUTANTS DEFECTIVE IN RECEPTOR-MEDIATED AND FLUID-PHASE ENDOCYTOSIS IN SACCHAROMYCES-CEREVISIAE [J].
RATHS, S ;
ROHRER, J ;
CRAUSAZ, F ;
RIEZMAN, H .
JOURNAL OF CELL BIOLOGY, 1993, 120 (01) :55-65
[35]   ENDOCYTOSIS IN YEAST - SEVERAL OF THE YEAST SECRETORY MUTANTS ARE DEFECTIVE IN ENDOCYTOSIS [J].
RIEZMAN, H .
CELL, 1985, 40 (04) :1001-1009
[36]   Ssp1 promotes actin depolymerization and is involved in stress response and new end take-off control in fission yeast [J].
Rupes, I ;
Jia, ZP ;
Young, PG .
MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (05) :1495-1510
[37]   Regulation of cell polarity by microtubules in fission yeast [J].
Sawin, KE ;
Nurse, P .
JOURNAL OF CELL BIOLOGY, 1998, 142 (02) :457-471
[38]   GENETIC-ANALYSIS OF CELL MORPHOGENESIS IN FISSION YEAST - A ROLE FOR CASEIN KINASE-II IN THE ESTABLISHMENT OF POLARIZED GROWTH [J].
SNELL, V ;
NURSE, P .
EMBO JOURNAL, 1994, 13 (09) :2066-2074
[39]   Single and multiple vesicle fusion induce different rates of endocytosis at a central synapse [J].
Sun, JY ;
Wu, XS ;
Wu, LG .
NATURE, 2002, 417 (6888) :555-559
[40]   Huntington's disease gene product, huntingtin, associates with microtubules in vitro [J].
Tukamoto, T ;
Nukina, N ;
Ide, K ;
Kanazawa, I .
MOLECULAR BRAIN RESEARCH, 1997, 51 (1-2) :8-14