The best control for the specificity of RNAi

被引：14

作者：

Sarov, M ^{[1
]}

Stewart, AF ^{[1
]}

机构：

[1] Dresden Univ Technol, BioInnovationZentrum, D-01307 Dresden, Germany

来源：

TRENDS IN BIOTECHNOLOGY | 2005年 / 23卷 / 09期

关键词：

D O I：

10.1016/j.tibtech.2005.06.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

RNA interference (RNAi) is revolutionizing functional genomics. However, there are several reasons to be concerned about the specificity and off-target effects of this technique. A recent paper by Kittler et al. describes a straightforward way to validate RNAi specificity, which exploits the increasing availability of bacterial artificial chromosome (BAC) clone resources. Genetic rescue of the RNAi phenotype by BAC transgenesis; is the best control yet described for specificity, and has further implications for reverse genetics.

引用

页码：446 / 448

页数：3

共 12 条

[1]

ANGRAND PO, 1999, NUCLEIC ACIDS RES, V27, P16

[2] Whither RNAi? [J].

不详 .

NATURE CELL BIOLOGY, 2003, 5 (06) :489-490

[3] Induction of an interferon response by RNAi vectors in mammalian cells [J].

Bridge, AJ ;

Pebernard, S ;

Ducraux, A ;

Nicoulaz, AL ;

Iggo, R .

NATURE GENETICS, 2003, 34 (03) :263-264

[4] Systematic genome-wide screens of gene function [J].

Carpenter, AE ;

Sabatini, DM .

NATURE REVIEWS GENETICS, 2004, 5 (01) :11-22

[5] Recombineering: A powerful new tool for mouse functional genomics [J].

Copeland, NG ;

Jenkins, NA ;

Court, DL .

NATURE REVIEWS GENETICS, 2001, 2 (10) :769-779

[6] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].

Elbashir, SM ;

Harborth, J ;

Lendeckel, W ;

Yalcin, A ;

Weber, K ;

Tuschl, T .

NATURE, 2001, 411 (6836) :494-498

[7] Unlocking the potential of the human genome with RNA interference [J].

Hannon, GJ ;

Rossi, JJ .

NATURE, 2004, 431 (7006) :371-378

[8] RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells [J].

Kittler, R ;

Pelletier, L ;

Ma, CL ;

Poser, I ;

Fischer, S ;

Hyman, AA ;

Buchholz, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (07) :2396-2401

[9] Mechanisms of gene silencing by double-stranded RNA [J].

Meister, G ;

Tuschl, T .

NATURE, 2004, 431 (7006) :343-349

[10] Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs) [J].

Persengiev, SP ;

Zhu, XC ;

Green, MR .

RNA, 2004, 10 (01) :12-18

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