A novel glycosaminoglycan-like polysaccharide from abalone Haliotis discus hannai Ino: Purification, structure identification and anticoagulant activity

被引：94

作者：

Li, Guoyun ^{[1
]}

Chen, Shiguo ^{[2
]}

Wang, Yuming ^{[1
]}

Xue, Yong ^{[1
]}

Chang, Yaoguang ^{[1
]}

Li, Zhaojie ^{[1
]}

Wang, Jingfeng ^{[1
]}

Xue, Changhu ^{[1
]}

机构：

[1] Ocean Univ China, Coll Food Sci & Technol, Qingdao 266003, Shandong, Peoples R China

[2] Zhejing Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310029, Zhejiang, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2011年 / 49卷 / 05期

基金：

对外科技合作项目（国际科技项目）;

关键词：

Abalone; Glycosaminoglycan; Purification; NMR analysis; Anticoagulant; FUCOSYLATED CHONDROITIN SULFATE; SEA-CUCUMBER;

D O I：

10.1016/j.ijbiomac.2011.09.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

A novel glycosaminoglycan-like sulfated polysaccharide (AAP) from the pleopods of Haliotis discus hannai Ino was purified by DEAE ion exchange chromatography followed with S-300 HR geltrion chromatography. Chemical composition analysis indicated that MP was composed of galactosamine, glucuronic acid, fucose, galactose with a ratio of 2.14:2.37:2.94:1, the content of sulfate was 15.5%. The average molecular weight (M-w) was 56.2 kDa in a TSK G4000 column. Further IR, 1D, 2D NMR spectroscopic analysis of the correlation signals of different sugar units gave a proposal repeating structure, as follows: [GRAPHICS] In vitro anticoagulant assay indicated AAP prolonged both the activated partial thromboplastin time (APT) and thrombin time (TT), with a 22.5 U/mg and 72.0 U/mg compared with standard heparin, respectively. The anticoagulant property of AAP was mainly attributed to powerful potentiation thrombin by anti-thrombin III. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1160 / 1166

页数：7

共 24 条

[21]

Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera [J].